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Potassium monopersulfate and a water-soluble manganese porphyrin complex, [Mn(TMPyP)](OAc)5, as an efficient reagent for the oxidative cleavage of DNA.
- Source :
-
Biochemistry [Biochemistry] 1989 Sep 05; Vol. 28 (18), pp. 7268-75. - Publication Year :
- 1989
-
Abstract
- Reported studies indicate that the association of potassium monopersulfate with [Mn(TMPyP)](OAc)5, a water-soluble manganese porphyrin complex, leads to an efficient reagent for the oxidative cleavage of DNA. Single-strand breaks (SSBs) are observed on double-stranded DNA at manganese porphyrin concentrations as low as 0.5 nM with a short incubation time of 1 min. The number of SSBs linearly varies with the concentration of the manganese complex, and potassium monopersulfate is at least 3 orders of magnitude more efficient as oxygen source than hydrogen peroxide. Cleavage efficiency is optimal in the pH range 7.5-9.0 for a NaCl concentration between 80 and 150 mM or for a MgCl2 concentration of 10 mM. At very low manganese porphyrin concentration and by increasing the incubation time a catalytic cleavage activity of the complex is evidenced: up to 5 SSBs per manganese porphyrin are observed. The high cleavage activity of the monopersulfate-manganese porphyrin system makes it a good candidate for DNA-footprinting experiments.
- Subjects :
- Chemical Phenomena
Chemistry, Physical
Electrophoresis, Agar Gel
Hydrogen Peroxide pharmacology
Hydrogen-Ion Concentration
Magnesium Chloride pharmacology
Oxidation-Reduction
Oxygen metabolism
Potassium
Sodium Chloride pharmacology
Solubility
Temperature
Time Factors
DNA
DNA, Single-Stranded
Potassium Compounds
Sulfates
Subjects
Details
- Language :
- English
- ISSN :
- 0006-2960
- Volume :
- 28
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2819067
- Full Text :
- https://doi.org/10.1021/bi00444a019