Back to Search
Start Over
Endocytosis contributes to BMP2-induced Smad signalling and neuronal growth.
- Source :
-
Neuroscience letters [Neurosci Lett] 2017 Mar 16; Vol. 643, pp. 32-37. Date of Electronic Publication: 2017 Feb 08. - Publication Year :
- 2017
-
Abstract
- Bone morphogenetic protein 2 (BMP2) is a neurotrophic factor which induces the growth of midbrain dopaminergic (DA) neurons in vitro and in vivo, and its neurotrophic effects have been shown to be dependent on activation of BMP receptors (BMPRs) and Smad 1/5/8 signalling. However, the precise intracellular cascades that regulate BMP2-BMPR-Smad-signalling-induced neurite growth remain unknown. Endocytosis has been shown to regulate Smad 1/5/8 signalling and differentiation induced by BMPs. However, these studies were carried out in non-neural cells. Indeed, there are scant reports regarding the role of endocytosis in BMP-Smad signalling in neurons. To address this, and to further characterise the mechanisms regulating the neurotrophic effects of BMP2, the present study examined the role of dynamin-dependent endocytosis in BMP2-induced Smad signalling and neurite growth in the SH-SY5Y neuronal cell line. The activation, temporal kinetics and magnitude of Smad 1/5/8 signalling induced by BMP2 were significantly attenuated by dynasore-mediated inhibition of endocytosis in SH-SY5Y cells. Furthermore, BMP2-induced increases in neurite length and neurite branching in SH-SY5Y cells were significantly reduced following inhibition of dynamin-dependent endocytosis using dynasore. This study demonstrates that BMP2-induced Smad signalling and neurite growth is regulated by dynamin-dependent endocytosis in a model of human midbrain dopaminergic neurons.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Apoptosis Regulatory Proteins
Bone Morphogenetic Protein 2 metabolism
Bone Morphogenetic Proteins metabolism
Cell Differentiation drug effects
Cell Differentiation physiology
Cell Line
Dopaminergic Neurons metabolism
Endocytosis physiology
Humans
Mesencephalon metabolism
Neurites drug effects
Neurites metabolism
Neurogenesis physiology
Bone Morphogenetic Protein 2 pharmacology
Endocytosis drug effects
Intracellular Signaling Peptides and Proteins metabolism
Mitochondrial Proteins metabolism
Neurogenesis drug effects
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7972
- Volume :
- 643
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 28188849
- Full Text :
- https://doi.org/10.1016/j.neulet.2017.02.013