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Gastroprotective and antielastase effects of protein inhibitors from Erythrina velutina seeds in an experimental ulcer model.
- Source :
-
Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2017 Apr; Vol. 95 (2), pp. 243-250. Date of Electronic Publication: 2016 Sep 12. - Publication Year :
- 2017
-
Abstract
- Trypsin and chymotrypsin inhibitors from Erythrina velutina seeds have been previously isolated by our group. In previous studies using a sepsis model, we demonstrated the antitumor and anti-inflammatory action of these compounds. This study aimed to evaluate the gastroprotective and antielastase effects of protein inhibitors from E. velutina seeds in an experimental stress-induced ulcer model. Two protein isolates from E. velutina seeds, with antitrypsin (PIAT) and antichymotrypsin (PIAQ) activities, were tested. Both protein isolates showed a high affinity and inhibitory effect against human neutrophil elastase, with 84% and 85% inhibition, respectively. Gastric ulcer was induced using ethanol (99%) in 6 groups of animals (female Wistar rats, n = 6). Before ulcer induction, these animals were treated for 5 days with one of the following: (1) PIAT (0.2 mg·kg <superscript>-1</superscript> ), (2) PIAT (0.4 mg·kg <superscript>-1</superscript> ), (3) PIAQ (0.035 mg·kg <superscript>-1</superscript> ), (4) ranitidine hydrochloride (50 mg·kg <superscript>-1</superscript> ), (5) saline solution (0.9%), or (6) no intervention (sham). Both PIAT and PIAQ protected gastric mucosa, preventing hemorrhagic lesions, edema, and mucus loss. No histologic toxic effects of PIAT or PIAQ were seen in liver and pancreatic cells. Our results show that protein isolates from E. velutina seeds have potential gastroprotective effects, placing these compounds as natural candidates for gastric ulcer prevention.
- Subjects :
- Animals
Anti-Inflammatory Agents isolation & purification
Disease Models, Animal
Enzyme Inhibitors isolation & purification
Ethanol
Female
Gastric Mucosa drug effects
Gastric Mucosa enzymology
Gastric Mucosa pathology
Gastrointestinal Agents isolation & purification
Humans
Leukocyte Elastase antagonists & inhibitors
Leukocyte Elastase metabolism
Plant Extracts chemistry
Ranitidine pharmacology
Rats
Rats, Wistar
Seeds chemistry
Stomach Ulcer chemically induced
Stomach Ulcer enzymology
Stomach Ulcer pathology
Anti-Inflammatory Agents pharmacology
Enzyme Inhibitors pharmacology
Erythrina chemistry
Gastrointestinal Agents pharmacology
Phytotherapy
Stomach Ulcer prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1208-6002
- Volume :
- 95
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemistry and cell biology = Biochimie et biologie cellulaire
- Publication Type :
- Academic Journal
- Accession number :
- 28177773
- Full Text :
- https://doi.org/10.1139/bcb-2016-0034