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In Vitro Sensitivity of Cutaneous Leishmania Promastigote Isolates Circulating in French Guiana to a Set of Drugs.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2017 May; Vol. 96 (5), pp. 1143-1150. Date of Electronic Publication: 2017 Feb 06. - Publication Year :
- 2017
-
Abstract
- AbstractAnti-leishmaniasis drug resistance is a common problem worldwide. The aim of this study was to inventory the general in vitro level of sensitivity of Leishmania isolates circulating in French Guiana and to highlight potential in vitro pentamidine-resistant isolates. This sensitivity study was conducted on 36 patient-promastigote isolates for seven drugs (amphotericin B, azithromycin, fluconazole, meglumine antimoniate, miltefosine, paromomycin, and pentamidine) using the Cell Counting Kit-8 viability test. The IC <subscript>50</subscript> values obtained were heterogeneous. One isolate exhibited high IC <subscript>50</subscript> values for almost all drugs tested. Pentamidine, which is the first-line treatment in French Guiana, showed efficacy at very low doses (mean of 0.0038 μg/mL). The concordance of the in vitro pentamidine results with the patients' clinical outcomes was 94% ( K = 0.82).
- Subjects :
- Amphotericin B pharmacology
Azithromycin pharmacology
Drug Resistance
Fluconazole pharmacology
Humans
Leishmania braziliensis growth & development
Leishmania braziliensis isolation & purification
Leishmania guyanensis growth & development
Leishmania guyanensis isolation & purification
Leishmaniasis, Cutaneous parasitology
Meglumine pharmacology
Meglumine Antimoniate
Organometallic Compounds pharmacology
Parasitic Sensitivity Tests
Paromomycin pharmacology
Phosphorylcholine analogs & derivatives
Phosphorylcholine pharmacology
Treatment Outcome
Antiprotozoal Agents pharmacology
Leishmania braziliensis drug effects
Leishmania guyanensis drug effects
Leishmaniasis, Cutaneous drug therapy
Pentamidine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1476-1645
- Volume :
- 96
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 28167598
- Full Text :
- https://doi.org/10.4269/ajtmh.16-0373