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Overexpression of Jagged1 Ameliorates Aged Rat-Derived Endothelial Progenitor Cell Functions and Improves Its Transfusion Efficiency for Rat Balloon-Induced Arterial Injury.
- Source :
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Annals of vascular surgery [Ann Vasc Surg] 2017 May; Vol. 41, pp. 241-258. Date of Electronic Publication: 2017 Feb 03. - Publication Year :
- 2017
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Abstract
- Background: Endothelial progenitor cell (EPC) has significant age-dependent alterations in properties, but the role of Jagged1 in aging-induced decline of EPC functions remains unclear.<br />Methods: 2- and 20-month old healthy male Sprague-Dawley rats were used in present study. Jagged1 gene transfection was performed in EPC isolated from aged (AEPC) and young rats (YEPC), respectively. Experiments were divided into 4 groups: (1) pIRES2-EGFP (PE) group, (2) PE-combined N-[N-(3, 5-difluoro-phenacetyl)-1- alany1]-S-phenyglycine t-butyl ester (DAPT) (PE + D) group, (3) pIRES2 EGFP-Jagged1 (PEJ) group, and (4) PEJ combined DAPT (PEJ + D) group. Notch molecules were detected by real-time quantitative polymerase chain reaction or Western blotting. CD34, CD133, CD45, and KDR markers were detected by flow cytometry. EPC migration and proliferation were detected with a modified Boyden chamber and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, respectively; the tube formation ability was assayed by in vitro angiogenesis kit; EPC transfusion after Jagged1 gene transfection was performed in rat carotid artery injury models.<br />Results: Jagged1 gene transfection effectively activates notch-signaling pathway. Compared with PE groups, overexpression of Jagged1 significantly promoted AEPC functions including proliferation, migration, the tube formation ability, and cell differentiation, these effects could be reasonably diminished by DAPT. In vivo study demonstrated that Jagged1 overexpressing also significantly promoted AEPC homing to the vascular injury sites and decreases the neointima formation after vascular injury.<br />Conclusions: Overexpression of Jagged1 ameliorates aged rat-derived EPC functions and increases its transfusion efficiency for balloon-induced rat arterial injury.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Subjects :
- Age Factors
Animals
Apoptosis
Carotid Artery Injuries metabolism
Carotid Artery Injuries pathology
Carotid Artery, Common pathology
Cell Movement
Cell Proliferation
Cells, Cultured
Endothelial Progenitor Cells metabolism
Jagged-1 Protein genetics
Male
Neointima
Rats, Sprague-Dawley
Signal Transduction
Time Factors
Transfection
Up-Regulation
Angioplasty, Balloon adverse effects
Carotid Artery Injuries surgery
Carotid Artery, Common metabolism
Endothelial Progenitor Cells transplantation
Jagged-1 Protein metabolism
Neovascularization, Physiologic
Subjects
Details
- Language :
- English
- ISSN :
- 1615-5947
- Volume :
- 41
- Database :
- MEDLINE
- Journal :
- Annals of vascular surgery
- Publication Type :
- Academic Journal
- Accession number :
- 28163178
- Full Text :
- https://doi.org/10.1016/j.avsg.2016.10.030