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Over-expression of SlJA2 decreased heat tolerance of transgenic tobacco plants via salicylic acid pathway.
- Source :
-
Plant cell reports [Plant Cell Rep] 2017 Apr; Vol. 36 (4), pp. 529-542. Date of Electronic Publication: 2017 Feb 02. - Publication Year :
- 2017
-
Abstract
- Key Message: Over-expression of SlJA2 decreased the accumulation of SA, which resulted in significant physiological and gene expression changes in transgenic tobacco plants, leading to the decreased heat tolerance of transgenic tobacco. NAC family, the largest transcription factors in plants, responses to different environmental stimuli. Here, we isolated a typical NAC transcription factor (SlJA2) from tomato and got transgenic tobacco with SlJA2 over-expression. Expression of SlJA2 was induced by heat stress (42 °C), chilling stress (4 °C), drought stress, osmotic stress, abscisic acid, and salicylic acid. Over-expression of SlJA2 decreased the accumulation of salicylic acid by regulating expression of salicylic acid degradation gene under heat stress. Compared to WT plants, stomatal apertures and water loss increased in transgenic plants, and the damage of photosynthetic apparatus and chlorophyll breakdown were more serious in transgenic plants under heat stress. Meanwhile, more H <subscript>2</subscript> O <subscript>2</subscript> and O <subscript>2</subscript> <superscript>·-</superscript> were accumulated transgenic plants and proline synthesis was restricted, which resulted in more serious oxidative damage compared to WT. qRT-PCR analysis showed that over-expression of SlJA2 could down-regulate genes involved in reactive oxygen species scavenging, proline biosynthesis, and response to heat stress. All the above results indicated that SlJA2 may be a negative regulator responded to plant's heat tolerance. Thus, this study provides new insight into roles of NAC family member in plant response to abiotic stress.
- Subjects :
- Droughts
Hydrogen Peroxide metabolism
Solanum lycopersicum genetics
Plant Proteins genetics
Plants, Genetically Modified drug effects
Plants, Genetically Modified metabolism
Reactive Oxygen Species metabolism
Salicylic Acid pharmacology
Signal Transduction drug effects
Stress, Physiological drug effects
Nicotiana drug effects
Nicotiana genetics
Gene Expression drug effects
Gene Expression genetics
Plant Proteins metabolism
Plants, Genetically Modified genetics
Signal Transduction genetics
Stress, Physiological genetics
Thermotolerance genetics
Nicotiana metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 36
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 28155114
- Full Text :
- https://doi.org/10.1007/s00299-017-2100-9