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Fcγ receptor expression on splenic macrophages in adult immune thrombocytopenia.
- Source :
-
Clinical and experimental immunology [Clin Exp Immunol] 2017 May; Vol. 188 (2), pp. 275-282. Date of Electronic Publication: 2017 Feb 28. - Publication Year :
- 2017
-
Abstract
- Splenic macrophages play a key role in immune thrombocytopenia (ITP) pathogenesis by clearing opsonized platelets. Fcγ receptors (FcγR) participate in this phenomenon, but their expression on splenic macrophages and their modulation by treatment have scarcely been studied in human ITP. We aimed to compare the phenotype and function of splenic macrophages between six controls and 24 ITP patients and between ITP patients according to the treatments they received prior to splenectomy. CD86, human leucocyte antigen D-related (HLA-DR) and FcγR expression were measured by flow cytometry on splenic macrophages. The major FcγR polymorphisms were determined and splenic macrophage function was assessed by a phagocytosis assay. The expression of the activation markers CD86 and HLA-DR was higher on splenic macrophages during ITP compared to controls. While the expression of FcγR was not different between ITP and controls, the phagocytic function of splenic macrophages was reduced in ITP patients treated with intravenous immunoglobulin (IVIg) within the 2 weeks prior to splenectomy. The FCGR3A (158V/F) polymorphism, known to increase the affinity of FcγRIII to IgG, was over-represented in ITP patients. Thus, these are the first results arguing for the fact that the therapeutic use of IVIg during human chronic ITP does not modulate FcγR expression on splenic macrophages but decreases their phagocytic capabilities.<br /> (© 2017 British Society for Immunology.)
- Subjects :
- Adult
Aged
Autoimmune Diseases surgery
Autoimmune Diseases therapy
B7-2 Antigen analysis
Female
Flow Cytometry
Humans
Immunoglobulin G blood
Immunoglobulins, Intravenous therapeutic use
Macrophages physiology
Male
Middle Aged
Phagocytosis
Phenotype
Polymorphism, Genetic
Receptors, IgG immunology
Spleen cytology
Splenectomy
Thrombocytopenia surgery
Thrombocytopenia therapy
Autoimmune Diseases immunology
Macrophages immunology
Receptors, IgG analysis
Receptors, IgG genetics
Spleen immunology
Thrombocytopenia immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2249
- Volume :
- 188
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinical and experimental immunology
- Publication Type :
- Academic Journal
- Accession number :
- 28142207
- Full Text :
- https://doi.org/10.1111/cei.12935