A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine.

Authors :: Fonseca EL
Ramos ND
Andrade BG
Morais LL
Marin MF
Vicente AC
Source :: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2017 Apr; Vol. 87 (4), pp. 315-317. Date of Electronic Publication: 2017 Jan 17.
Publication Year :: 2017
Abstract: Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for bla <subscript>SHV</subscript> , bla <subscript>LEN</subscript> and bla <subscript>OKP</subscript> and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.<br /> (Copyright © 2017 Elsevier Inc. All rights reserved.)

Subjects :: DNA, Bacterial genetics
Humans
Microbial Sensitivity Tests methods
beta-Lactamases genetics
Klebsiella genetics
Klebsiella Infections microbiology
Klebsiella pneumoniae genetics
Multiplex Polymerase Chain Reaction methods

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