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Fibrinolysis inhibitors in plaque stability: a morphological association of PAI-1 and TAFI in advanced carotid plaque.
- Source :
-
Journal of thrombosis and haemostasis : JTH [J Thromb Haemost] 2017 Apr; Vol. 15 (4), pp. 758-769. Date of Electronic Publication: 2017 Feb 28. - Publication Year :
- 2017
-
Abstract
- Essentials Fibrinolysis inhibitors are localized in advanced atheroma by immunohistology of endarterectomies. Neovascular endothelium/neocapillaries show thrombin-activatable fibrinolysis inhibitor (TAFI). Macrophage areas show free plasminogen activator inhibitor (PAI-1), notably in the vulnerable part. Free PAI-1 and TAFI stabilize active plaque area by inhibition of fibrinolysis and inflammation.<br />Summary: Background Fibrinolysis plays an important role in destabilization of atherosclerotic plaques and is tightly regulated by specific inhibitors. Objective The fibrinolysis inhibitors plasminogen activator inhibitor type-1 (PAI-1) and thrombin-activatable fibrinolysis inhibitor (TAFI) were quantified and described in the morphological context of advanced carotid plaques American Heart Association VI-VIII to elucidate their role in plaque stability. Methods Immunohistochemistry in serial sections along the longitudinal axis of endarterectomies from patients with symptomatic carotid stenosis (n = 19) were studied using an antibody specific for free PAI-1 (I205), an antibody with high affinity for TAFI/TAFIa (CP17) and established antibodies for smooth muscle cells (α-actin), endothelial cells (von Willebrand factor [VWF]), macrophages (CD68) and platelets (CD42). Results PAI-1 and TAFI show a specific distribution in these advanced plaques with a maximum corresponding to the internal carotid artery (ICA). Free PAI-1 was mainly detected in macrophages and in intravascular thrombi, and TAFI in endothelial cells (ECs) but also macrophages. The one-way ANOVA analysis with Bonferroni's correction showed a significant increase of macrophages and ECs, TAFI and PAI-1 in areas with high neovascularization in endarterectomy sections corresponding to ICA. High Spearman factors for TAFI, PAI-1 and VWF indicate neovascularization as the main source of plasma proteins, transported by platelets into the atheroma (PAI-1) or expressed by ECs (TAFI). CD68 was highly associated with VWF, PAI-1 and especially TAFI, underlining the role of macrophages in fibrinolytic activity and inflammation. Conclusion The abundance of free PAI-1 and TAFI in the plaque may inhibit plasmin generation and thereby counteract plaque destabilization by fibrinolysis, cell migration and inflammation.<br /> (© 2017 International Society on Thrombosis and Haemostasis.)
- Subjects :
- Aged
Anticoagulants pharmacology
Antigens, CD metabolism
Antigens, Differentiation, Myelomonocytic metabolism
Carotid Arteries pathology
Endarterectomy
Female
Fibrinogen pharmacology
Fibrinolysin pharmacology
Humans
Immunohistochemistry
Inflammation
Macrophages metabolism
Male
Middle Aged
Myocytes, Smooth Muscle metabolism
Pilot Projects
Plaque, Atherosclerotic pathology
Platelet Glycoprotein GPIb-IX Complex metabolism
Thrombin pharmacology
Thrombosis
von Willebrand Factor metabolism
Carboxypeptidase B2 metabolism
Carotid Stenosis pathology
Fibrinolysis drug effects
Plasminogen Activator Inhibitor 1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1538-7836
- Volume :
- 15
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of thrombosis and haemostasis : JTH
- Publication Type :
- Academic Journal
- Accession number :
- 28135035
- Full Text :
- https://doi.org/10.1111/jth.13641