Design of a Bioabsorbable Multilayered Patch for Esophagus Tissue Engineering.

Authors :: Dorati R
De Trizio A
Marconi S
Ferrara A
Auricchio F
Genta I
Modena T
Benazzo M
Benazzo A
Volpato G
Conti B
Source :: Macromolecular bioscience [Macromol Biosci] 2017 Jun; Vol. 17 (6). Date of Electronic Publication: 2017 Jan 27.
Publication Year :: 2017
Abstract: A gold standard for esophagus reconstruction is not still available. The present work aims to design a polymer patch combining synthetic polylactide-co-polycaprolacton and chitosan biopolymers, tailoring patch properties to esophageal tissue characteristics by a temperature-induced precipitation method, to get multilayered patches (1L, 2L, and 3L). Characterization shows stable multilayered patches (1L and 2L) by selection of copolymer type, and their M <subscript>w</subscript> . In vitro investigation of the functional patch properties in simulated physiologic and pathologic conditions demonstrates that the chitosan layer (patch 3L) decreases patch stability and cell adhesion, while improves cell proliferation. Patches 2L and 3L comply with physiological esophageal pressure (3-5 kPa) and elongation (20%).<br /> (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)