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Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication.
- Source :
-
Scientific reports [Sci Rep] 2017 Jan 27; Vol. 7, pp. 41389. Date of Electronic Publication: 2017 Jan 27. - Publication Year :
- 2017
-
Abstract
- Zika virus (ZIKV) is an emerging mosquito-borne (Aedes genus) arbovirus of the Flaviviridae family. Although ZIKV has been predominately associated with a mild or asymptomatic dengue-like disease, its appearance in the Americas has been accompanied by a multi-fold increase in reported incidence of fetal microcephaly and brain malformations. The source and mode of vertical transmission from mother to fetus is presumptively transplacental, although a causal link explaining the interval delay between maternal symptoms and observed fetal malformations following infection has been missing. In this study, we show that primary human placental trophoblasts from non-exposed donors (nā=ā20) can be infected by primary passage ZIKV-FLR isolate, and uniquely allowed for ZIKV viral RNA replication when compared to dengue virus (DENV). Consistent with their being permissive for ZIKV infection, primary trophoblasts expressed multiple putative ZIKV cell entry receptors, and cellular function and differentiation were preserved. These findings suggest that ZIKV-FLR strain can replicate in human placental trophoblasts without host cell destruction, thereby serving as a likely permissive reservoir and portal of fetal transmission with risk of latent microcephaly and malformations.<br />Competing Interests: The authors declare no competing financial interests.
- Subjects :
- Adult
Cells, Cultured
Dengue pathology
Dengue virology
Dengue Virus physiology
Female
Giant Cells metabolism
Giant Cells pathology
Humans
Ligands
MicroRNAs genetics
MicroRNAs metabolism
Phylogeny
Pregnancy
RNA, Viral metabolism
Receptors, Virus metabolism
Toll-Like Receptors metabolism
Zika Virus Infection pathology
Zika Virus Infection virology
Placenta pathology
Trophoblasts virology
Virus Replication physiology
Zika Virus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 28128342
- Full Text :
- https://doi.org/10.1038/srep41389