Back to Search
Start Over
Detection of vulnerable neurons damaged by environmental insults in utero.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2017 Feb 28; Vol. 114 (9), pp. 2367-2372. Date of Electronic Publication: 2017 Jan 25. - Publication Year :
- 2017
-
Abstract
- Development of prognostic biomarkers for the detection of prenatally damaged neurons before manifestations of postnatal disorders is an essential step for prevention and treatment of susceptible individuals. We have developed a versatile fluorescence reporter system in mice enabling detection of Heat Shock Factor 1 activation in response to prenatal cellular damage caused by exposure to various harmful chemical or physical agents. Using an intrautero electroporation-mediated reporter assay and transgenic reporter mice, we are able to identify neurons that survive prenatal exposure to harmful agents but remain vulnerable in postnatal life. This system may provide a powerful tool for exploring the pathogenesis and treatment of multiple disorders caused by exposure to environmental stress before symptoms become manifested, exacerbated, and/or irreversible.
- Subjects :
- Animals
Cerebral Cortex metabolism
Cerebral Cortex pathology
Electroporation
Embryo, Mammalian
Ethanol toxicity
Female
Flow Cytometry
Gene Expression Regulation
Genes, Reporter
Heat Shock Transcription Factors metabolism
Heat-Shock Response genetics
Luminescent Proteins genetics
Luminescent Proteins metabolism
Mice
Mice, Transgenic
Neurons metabolism
Neurons pathology
Nicotine toxicity
Plasmids chemistry
Plasmids metabolism
Pregnancy
Prenatal Exposure Delayed Effects chemically induced
Prenatal Exposure Delayed Effects pathology
Suramin toxicity
Cerebral Cortex drug effects
Heat Shock Transcription Factors genetics
Neurons drug effects
Prenatal Exposure Delayed Effects diagnosis
Prenatal Exposure Delayed Effects genetics
Response Elements
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 114
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 28123061
- Full Text :
- https://doi.org/10.1073/pnas.1620641114