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Bicarbonate-sensing soluble adenylyl cyclase is present in the cell cytoplasm and nucleus of multiple shark tissues.
- Source :
-
Physiological reports [Physiol Rep] 2017 Jan; Vol. 5 (2). - Publication Year :
- 2017
-
Abstract
- The enzyme soluble adenylyl cyclase (sAC) is directly stimulated by bicarbonate (HCO <subscript>3</subscript> <superscript>-</superscript> ) to produce the signaling molecule cyclic adenosine monophosphate (cAMP). Because sAC and sAC-related enzymes are found throughout phyla from cyanobacteria to mammals and they regulate cell physiology in response to internal and external changes in pH, CO <subscript>2</subscript> , and HCO <subscript>3</subscript> <superscript>-</superscript> , sAC is deemed an evolutionarily conserved acid-base sensor. Previously, sAC has been reported in dogfish shark and round ray gill cells, where they sense and counteract blood alkalosis by regulating the activity of V-type H <superscript>+</superscript> - ATPase. Here, we report the presence of sAC protein in gill, rectal gland, cornea, intestine, white muscle, and heart of leopard shark Triakis semifasciata Co-expression of sAC with transmembrane adenylyl cyclases supports the presence of cAMP signaling microdomains. Furthermore, immunohistochemistry on tissue sections, and western blots and cAMP-activity assays on nucleus-enriched fractions demonstrate the presence of sAC protein in and around nuclei. These results suggest that sAC modulates multiple physiological processes in shark cells, including nuclear functions.<br /> (© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.)
Details
- Language :
- English
- ISSN :
- 2051-817X
- Volume :
- 5
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Physiological reports
- Publication Type :
- Academic Journal
- Accession number :
- 28108644
- Full Text :
- https://doi.org/10.14814/phy2.13090