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Evaluation of multiplex assay platforms for detection of influenza hemagglutinin subtype specific antibody responses.
- Source :
-
Journal of virological methods [J Virol Methods] 2017 May; Vol. 243, pp. 61-67. Date of Electronic Publication: 2017 Jan 17. - Publication Year :
- 2017
-
Abstract
- Influenza hemagglutination inhibition (HI) and virus microneutralization assays (MN) are widely used for seroprevalence studies. However, these assays have limited field portability and are difficult to fully automate for high throughput laboratory testing. To address these issues, three multiplex influenza subtype-specific antibody detection assays were developed using recombinant hemagglutinin antigens in combination with Chembio, Luminex <superscript>®</superscript> , and ForteBio <superscript>®</superscript> platforms. Assay sensitivity, specificity, and subtype cross-reactivity were evaluated using a panel of well characterized human sera. Compared to the traditional HI, assay sensitivity ranged from 87% to 92% and assay specificity in sera collected from unexposed persons ranged from 65% to 100% across the platforms. High assay specificity (86-100%) for A(H5N1) rHA was achieved for sera from exposed or unexposed to hetorosubtype influenza HAs. In contrast, assay specificity for A(H1N1)pdm09 rHA using sera collected from A/Vietnam/1204/2004 (H5N1) vaccinees in 2008 was low (22-30%) in all platforms. Although cross-reactivity against rHA subtype proteins was observed in each assay platform, the correct subtype specific responses were identified 78%-94% of the time when paired samples were available for analysis. These results show that high throughput and portable multiplex assays that incorporate rHA can be used to identify influenza subtype specific infections.<br /> (Published by Elsevier B.V.)
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Child
Child, Preschool
Humans
Middle Aged
Sensitivity and Specificity
Young Adult
Antibodies, Viral blood
Hemagglutinin Glycoproteins, Influenza Virus immunology
High-Throughput Screening Assays methods
Influenza, Human epidemiology
Influenza, Human virology
Serologic Tests methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0984
- Volume :
- 243
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 28108183
- Full Text :
- https://doi.org/10.1016/j.jviromet.2017.01.008