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Inhibition of Fatty Acid Synthase Reduces Blastocyst Hatching through Regulation of the AKT Pathway in Pigs.
- Source :
-
PloS one [PLoS One] 2017 Jan 20; Vol. 12 (1), pp. e0170624. Date of Electronic Publication: 2017 Jan 20 (Print Publication: 2017). - Publication Year :
- 2017
-
Abstract
- Fatty acid synthase (FASN) is an enzyme responsible for the de novo synthesis of long-chain fatty acids. During oncogenesis, FASN plays a role in growth and survival rather than acting within the energy storage pathways. Here, the function of FASN during early embryonic development was studied using its specific inhibitor, C75. We found that the presence of the inhibitor reduced blastocyst hatching. FASN inhibition decreased Cpt1 expression, leading to a reduction in mitochondria numbers and ATP content. This inhibition of FASN resulted in the down-regulation of the AKT pathway, thereby triggering apoptosis through the activation of the p53 pathway. Activation of the apoptotic pathway also leads to increased accumulation of reactive oxygen species and autophagy. In addition, the FASN inhibitor impaired cell proliferation, a parameter of blastocyst quality for outgrowth. The level of OCT4, an important factor in embryonic development, decreased after treatment with the FASN inhibitor. These results show that FASN exerts an effect on early embryonic development by regulating both fatty acid oxidation and the AKT pathway in pigs.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Cell Proliferation drug effects
Cell Proliferation physiology
Down-Regulation
Fatty Acid Synthases metabolism
Fatty Acid Synthases physiology
Fluorescent Antibody Technique
MicroRNAs metabolism
Microscopy, Confocal
Reactive Oxygen Species metabolism
Real-Time Polymerase Chain Reaction
Signal Transduction drug effects
Swine embryology
Blastocyst physiology
Fatty Acid Synthases antagonists & inhibitors
Oncogene Protein v-akt physiology
Signal Transduction physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 28107461
- Full Text :
- https://doi.org/10.1371/journal.pone.0170624