Photocontrolled extraction of uric acid from biological samples based on photoresponsive surface molecularly imprinted polymer microspheres.

Authors :: Gong CB
Li ZY
Liu LT
Wei YB
Yang X
Chow CF
Tang Q
Source :: Journal of separation science [J Sep Sci] 2017 Mar; Vol. 40 (6), pp. 1396-1402. Date of Electronic Publication: 2017 Feb 22.
Publication Year :: 2017
Abstract: We aim to develop novel photoresponsive surface molecularly imprinted polymer (SIMP) microspheres, an SiO <subscript>2</subscript> -SIMP, for the photocontrolled extraction of uric acid from biological samples. The SiO <subscript>2</subscript> -SMIP was prepared on silica microspheres by surface polymerization and characterized by using scanning electron microscopy, transmission electron microscopy, FTIR spectroscopy, thermogravimetric analysis, nitrogen adsorption-desorption analysis, and UV-visible spectroscopy. The SiO <subscript>2</subscript> -SMIP microspheres showed a photocontrolled uptake and release of uric acid in NaH <subscript>2</subscript> PO <subscript>4</subscript> buffer upon alternate irradiation at 365 and 440 nm. The SiO <subscript>2</subscript> -SMIP microspheres were able to photocontrollably extract uric acid from complicated biological samples for concentration analysis with no significant interference encountered and it exhibited very good recognition ability and fast binding kinetics toward uric acid.<br /> (© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Adsorption
Humans
Polymers
Silicon Dioxide
Uric Acid urine
Microspheres
Molecular Imprinting
Uric Acid isolation & purification

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