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Exposure to GDNF Enhances the Ability of Enteric Neural Progenitors to Generate an Enteric Nervous System.
- Source :
-
Stem cell reports [Stem Cell Reports] 2017 Feb 14; Vol. 8 (2), pp. 476-488. Date of Electronic Publication: 2017 Jan 12. - Publication Year :
- 2017
-
Abstract
- Cell therapy is a promising approach to generate an enteric nervous system (ENS) and treat enteric neuropathies. However, for translation to the clinic, it is highly likely that enteric neural progenitors will require manipulation prior to transplantation to enhance their ability to migrate and generate an ENS. In this study, we examine the effects of exposure to several factors on the ability of ENS progenitors, grown as enteric neurospheres, to migrate and generate an ENS. Exposure to glial-cell-line-derived neurotrophic factor (GDNF) resulted in a 14-fold increase in neurosphere volume and a 12-fold increase in cell number. Following co-culture with embryonic gut or transplantation into the colon of postnatal mice in vivo, cells derived from GDNF-treated neurospheres showed a 2-fold increase in the distance migrated compared with controls. Our data show that the ability of enteric neurospheres to generate an ENS can be enhanced by exposure to appropriate factors.<br /> (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Biomarkers
Cell Count
Cell Movement
Cell Proliferation
Cell Size drug effects
Cells, Cultured
Coculture Techniques
Glial Cell Line-Derived Neurotrophic Factor pharmacology
Mice
Mice, Transgenic
Neural Stem Cells drug effects
Neurons cytology
Neurons metabolism
Phenotype
Stem Cell Transplantation
Cell Differentiation drug effects
Enteric Nervous System cytology
Enteric Nervous System embryology
Glial Cell Line-Derived Neurotrophic Factor metabolism
Neural Stem Cells cytology
Neural Stem Cells metabolism
Neurogenesis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2213-6711
- Volume :
- 8
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Stem cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 28089669
- Full Text :
- https://doi.org/10.1016/j.stemcr.2016.12.013