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Functional characterisation and cell specificity of BvSUT1, the transporter that loads sucrose into the phloem of sugar beet (Beta vulgaris L.) source leaves.
- Source :
-
Plant biology (Stuttgart, Germany) [Plant Biol (Stuttg)] 2017 May; Vol. 19 (3), pp. 315-326. Date of Electronic Publication: 2017 Feb 01. - Publication Year :
- 2017
-
Abstract
- Sugar beet (Beta vulgaris L.) is one of the most important sugar-producing plants worldwide and provides about one third of the sugar consumed by humans. Here we report on molecular characterisation of the BvSUT1 gene and on the functional characterisation of the encoded transporter. In contrast to the recently identified tonoplast-localised sucrose transporter BvTST2.1 from sugar beet taproots, which evolved within the monosaccharide transporter (MST) superfamily, BvSUT1 represents a classical sucrose transporter and is a typical member of the disaccharide transporter (DST) superfamily. Transgenic Arabidopsis plants expressing the β-GLUCURONIDASE (GUS) reporter gene under control of the BvSUT1-promoter showed GUS histochemical staining of their phloem; an anti-BvSUT1-antiserum identified the BvSUT1 transporter specifically in phloem companion cells. After expression of BvSUT1 cDNA in bakers' yeasts (Saccharomyces cerevisiae) uptake characteristics of the BvSUT1 protein were studied. Moreover, the sugar beet transporter was characterised as a proton-coupled sucrose symporter in Xenopus laevis oocytes. Our findings indicate that BvSUT1 is the sucrose transporter that is responsible for loading of sucrose into the phloem of sugar beet source leaves delivering sucrose to the storage tissue in sugar beet taproot sinks.<br /> (© 2017 German Botanical Society and The Royal Botanical Society of the Netherlands.)
- Subjects :
- Animals
Arabidopsis genetics
Arabidopsis metabolism
Beta vulgaris genetics
Female
Gene Expression Regulation, Plant
Glucuronidase genetics
Glucuronidase metabolism
Membrane Transport Proteins genetics
Oocytes metabolism
Phloem metabolism
Plant Proteins genetics
Plants, Genetically Modified
Promoter Regions, Genetic
Saccharomyces cerevisiae genetics
Xenopus laevis
Beta vulgaris metabolism
Membrane Transport Proteins metabolism
Plant Leaves metabolism
Plant Proteins metabolism
Sucrose metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1438-8677
- Volume :
- 19
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Plant biology (Stuttgart, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 28075052
- Full Text :
- https://doi.org/10.1111/plb.12546