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Prophage-mediated defence against viral attack and viral counter-defence.

Prophage-mediated defence against viral attack and viral counter-defence.

Authors :
Dedrick RM
Jacobs-Sera D
Bustamante CA
Garlena RA
Mavrich TN
Pope WH
Reyes JC
Russell DA
Adair T
Alvey R
Bonilla JA
Bricker JS
Brown BR
Byrnes D
Cresawn SG
Davis WB
Dickson LA
Edgington NP
Findley AM
Golebiewska U
Grose JH
Hayes CF
Hughes LE
Hutchison KW
Isern S
Johnson AA
Kenna MA
Klyczek KK
Mageeney CM
Michael SF
Molloy SD
Montgomery MT
Neitzel J
Page ST
Pizzorno MC
Poxleitner MK
Rinehart CA
Robinson CJ
Rubin MR
Teyim JN
Vazquez E
Ware VC
Washington J
Hatfull GF
Source :
Nature microbiology [Nat Microbiol] 2017 Jan 09; Vol. 2, pp. 16251. Date of Electronic Publication: 2017 Jan 09.
Publication Year :
2017

Abstract

Temperate phages are common, and prophages are abundant residents of sequenced bacterial genomes. Mycobacteriophages are viruses that infect mycobacterial hosts including Mycobacterium tuberculosis and Mycobacterium smegmatis, encompass substantial genetic diversity and are commonly temperate. Characterization of ten Cluster N temperate mycobacteriophages revealed at least five distinct prophage-expressed viral defence systems that interfere with the infection of lytic and temperate phages that are either closely related (homotypic defence) or unrelated (heterotypic defence) to the prophage. Target specificity is unpredictable, ranging from a single target phage to one-third of those tested. The defence systems include a single-subunit restriction system, a heterotypic exclusion system and a predicted (p)ppGpp synthetase, which blocks lytic phage growth, promotes bacterial survival and enables efficient lysogeny. The predicted (p)ppGpp synthetase coded by the Phrann prophage defends against phage Tweety infection, but Tweety codes for a tetrapeptide repeat protein, gp54, which acts as a highly effective counter-defence system. Prophage-mediated viral defence offers an efficient mechanism for bacterial success in host-virus dynamics, and counter-defence promotes phage co-evolution.

Details

Language :
English
ISSN :
2058-5276
Volume :
2
Database :
MEDLINE
Journal :
Nature microbiology
Publication Type :
Academic Journal
Accession number :
28067906
Full Text :
https://doi.org/10.1038/nmicrobiol.2016.251