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Glycated albumin modifies platelet adhesion and aggregation responses.
- Source :
-
Platelets [Platelets] 2017 Nov; Vol. 28 (7), pp. 682-690. Date of Electronic Publication: 2017 Jan 09. - Publication Year :
- 2017
-
Abstract
- A diabetic vasculature is detrimental to cardiovascular health through the actions of advanced glycation end products (AGEs) on endothelial cells and platelets. Platelets activated by AGEs agonize endothelial responses promoting cardiovascular disease (CVD) development. While it has been established that AGEs can alter platelet functions, little is known about the specific platelet pathways that AGEs modify. Therefore, we evaluated the effects of AGEs on specific salient platelet pathways related to CVDs and whether the effects that AGEs elicit are dependent on glycation extent. To accomplish our objective, platelets were incubated with reversibly or irreversibly glycated albumin. A time course for adhesion and aggregation agonist receptor expression was assessed. Optical platelet aggregometry was used to confirm the functional activity of platelets after AGE exposure. In general, platelets subjected to glycated albumin had a significantly enhanced adhesion and aggregation potential. Furthermore, we observed an enhancement in dense body secretion and intracellular calcium concentration. This was especially prevalent for platelets exposed to irreversibly glycated albumin. Additionally, functional aggregation correlated well with receptor expression, suggesting that AGE-induced altered receptor sensitivity translated to altered platelet functions. Our findings indicate that under diabetic vascular conditions platelets become more susceptible to activation and aggregation due to an overall enhanced receptor expression, which may act to promote CVD development.
- Subjects :
- Adenosine Diphosphate pharmacology
Arachidonic Acid pharmacology
Blood Platelets cytology
Blood Platelets metabolism
Calcium metabolism
Cells, Cultured
Epinephrine pharmacology
Glycation End Products, Advanced
Humans
Platelet Function Tests
Receptors, Adrenergic, alpha-1 genetics
Receptors, Adrenergic, alpha-1 metabolism
Receptors, Purinergic P2Y1 genetics
Receptors, Purinergic P2Y1 metabolism
Receptors, Purinergic P2Y12 genetics
Receptors, Purinergic P2Y12 metabolism
Receptors, Thromboxane A2, Prostaglandin H2 genetics
Receptors, Thromboxane A2, Prostaglandin H2 metabolism
Glycated Serum Albumin
Blood Platelets drug effects
Gene Expression Regulation drug effects
Platelet Adhesiveness drug effects
Platelet Aggregation drug effects
Serum Albumin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1369-1635
- Volume :
- 28
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Platelets
- Publication Type :
- Academic Journal
- Accession number :
- 28067098
- Full Text :
- https://doi.org/10.1080/09537104.2016.1260703