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Mechanisms involved in antinociception induced by a polysulfated fraction from seaweed Gracilaria cornea in the temporomandibular joint of rats.
- Source :
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International journal of biological macromolecules [Int J Biol Macromol] 2017 Apr; Vol. 97, pp. 76-84. Date of Electronic Publication: 2017 Jan 05. - Publication Year :
- 2017
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Abstract
- Temporomandibular disorder is a common clinical condition involving pain in the temporomandibular joint (TMJ) region. This study assessed the antinociceptive effects of a polysulfated fraction from the red seaweed Gracilaria cornea (Gc-FI) on the formalin-induced TMJ hypernociception in rats and investigated the involvement of different mechanisms. Male Wistar rats were pretreated with injection (sc) of saline or Gc-FI 1h before intra- TMJ injection of formalin to evaluate the nociception. The results showed that pretreatment with Gc-FI significantly reduced formalin-induced nociceptive behavior. Moreover, the antinociceptive effect of the Gc-FI was blocked by naloxone (a non-selective opioid antagonist), suggesting the involvement of opioids selective receptors. Thus, the pretreatment with selective opioids receptors antagonists, reversed the antinociceptive effect of the Gc-FI in the TMJ. The Gc-FI antinociceptive effect depends on the nitric oxide/cyclic GMP/protein kinase G/ATP-sensitive potassium channel (NO/cGMP/PKG/K <superscript>+</superscript> <subscript>ATP</subscript> ) pathway because it was prevented by pretreatment with inhibitors of nitric oxide synthase, guanylate cyclase enzyme, PKG and a K <superscript>+</superscript> <subscript>ATP</subscript> blocker. In addition, after inhibition with a specific heme oxygenase-1 (HO-1) inhibitor, the antinociceptive effect of the Gc-FI was not observed. Collectively, these data suggest that the antinociceptive effect induced by Gc-FI is mediated by μ/δ/κ-opioid receptors and by activation NO/cGMP/PKG/K <superscript>+</superscript> <subscript>ATP</subscript> channel pathway, besides of HO-1.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Analgesics chemistry
Analgesics isolation & purification
Analgesics pharmacology
Animals
Cyclic GMP metabolism
Cyclic GMP-Dependent Protein Kinases metabolism
Formaldehyde pharmacology
Heme Oxygenase-1 metabolism
Interleukin-10 metabolism
KATP Channels metabolism
Male
Nociception drug effects
Plant Extracts isolation & purification
Rats
Rats, Wistar
Receptors, Opioid metabolism
Serotonin pharmacology
Signal Transduction drug effects
Temporomandibular Joint cytology
Temporomandibular Joint metabolism
Trigeminal Ganglion drug effects
Trigeminal Ganglion metabolism
Gracilaria chemistry
Plant Extracts chemistry
Plant Extracts pharmacology
Seaweed chemistry
Sulfates chemistry
Temporomandibular Joint drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 97
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 28065754
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2017.01.017