Back to Search
Start Over
Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats.
- Source :
-
In vivo (Athens, Greece) [In Vivo] 2017 Jan 02; Vol. 31 (1), pp. 45-50. - Publication Year :
- 2017
-
Abstract
- Background/aim: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.<br />Materials and Methods: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10 <superscript>3</superscript> S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH <subscript>2</subscript> and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.<br />Results/conclusion: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica <superscript>+</superscript> ) but not ica <superscript>-</superscript> S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica <superscript>+</superscript> or ica <superscript>-</superscript> S. aureus, suggesting a lack of specificity in this antiserum.<br /> (Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Subjects :
- Animals
Biomarkers analysis
Male
Osteomyelitis immunology
Osteomyelitis microbiology
Periprosthetic Fractures immunology
Periprosthetic Fractures microbiology
Rabbits
Rats
Rats, Sprague-Dawley
Staphylococcal Infections immunology
Staphylococcal Infections microbiology
Staphylococcus aureus pathogenicity
Immunization methods
Osteomyelitis prevention & control
Periprosthetic Fractures prevention & control
Staphylococcal Infections prevention & control
Staphylococcus aureus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7549
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- In vivo (Athens, Greece)
- Publication Type :
- Academic Journal
- Accession number :
- 28064219
- Full Text :
- https://doi.org/10.21873/invivo.11023