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Biodegradable nanoparticle delivery of inactivated swine influenza virus vaccine provides heterologous cell-mediated immune response in pigs.
- Source :
-
Journal of controlled release : official journal of the Controlled Release Society [J Control Release] 2017 Feb 10; Vol. 247, pp. 194-205. Date of Electronic Publication: 2017 Jan 02. - Publication Year :
- 2017
-
Abstract
- Swine influenza virus (SwIV) is one of the important zoonotic pathogens. Current flu vaccines have failed to provide cross-protection against evolving viruses in the field. Poly(lactic-co-glycolic acid) (PLGA) is a biodegradable FDA approved polymer and widely used in drug and vaccine delivery. In this study, inactivated SwIV H1N2 antigens (KAg) encapsulated in PLGA nanoparticles (PLGA-KAg) were prepared, which were spherical in shape with 200 to 300nm diameter, and induced maturation of antigen presenting cells in vitro. Pigs vaccinated twice with PLGA-KAg via intranasal route showed increased antigen specific lymphocyte proliferation and enhanced the frequency of T-helper/memory and cytotoxic T cells (CTLs) in peripheral blood mononuclear cells (PBMCs). In PLGA-KAg vaccinated and heterologous SwIV H1N1 challenged pigs, clinical flu symptoms were absent, while the control pigs had fever for four days. Grossly and microscopically, reduced lung pathology and viral antigenic mass in the lung sections with clearance of infectious challenge virus in most of the PLGA-KAg vaccinated pig lung airways were observed. Immunologically, PLGA-KAg vaccine irrespective of not significantly boosting the mucosal antibody response, it augmented the frequency of IFN-γ secreting total T cells, T-helper and CTLs against both H1N2 and H1N1 SwIV. In summary, inactivated influenza virus delivered through PLGA-NPs reduced the clinical disease and induced cross-protective cell-mediated immune response in a pig model. Our data confirmed the utility of a pig model for intranasal particulate flu vaccine delivery platform to control flu in humans.<br /> (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Subjects :
- Administration, Intranasal
Animals
Antigens, Viral immunology
Antigens, Viral therapeutic use
Cells, Cultured
Dogs
Immunity, Cellular
Influenza Vaccines immunology
Influenza Vaccines therapeutic use
Madin Darby Canine Kidney Cells
Orthomyxoviridae Infections immunology
Polylactic Acid-Polyglycolic Acid Copolymer
Swine
Vaccines, Inactivated immunology
Vaccines, Inactivated therapeutic use
Antigens, Viral administration & dosage
Influenza A Virus, H1N1 Subtype immunology
Influenza Vaccines administration & dosage
Lactic Acid chemistry
Nanoparticles chemistry
Orthomyxoviridae Infections prevention & control
Polyglycolic Acid chemistry
Vaccines, Inactivated administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4995
- Volume :
- 247
- Database :
- MEDLINE
- Journal :
- Journal of controlled release : official journal of the Controlled Release Society
- Publication Type :
- Academic Journal
- Accession number :
- 28057521
- Full Text :
- https://doi.org/10.1016/j.jconrel.2016.12.039