Back to Search
Start Over
In situ quantification of diverse titanium dioxide nanoparticles unveils selective endoplasmic reticulum stress-dependent toxicity.
- Source :
-
Nanotoxicology [Nanotoxicology] 2017 Feb; Vol. 11 (1), pp. 134-145. - Publication Year :
- 2017
-
Abstract
- Although titanium dioxide nanoparticles (TiO <subscript>2</subscript> NPs) have been extensively studied, their possible impact on health due to their specific properties supported by their size and geometry, remains to be fully characterized to support risk assessment. To further document NPs biological effects, we investigated the impact of TiO <subscript>2</subscript> NPs morphology on biological outcomes. To this end, TiO <subscript>2</subscript> NPs were synthesized as nanoneedles (NNs), titanate scrolled nanosheets (TNs), gel-sol-based isotropic nanoparticles (INPs) and tested for perturbation of cellular homeostasis (cellular ion content, cell proliferation, stress pathways) in three cell types and compared to the P25. We showed that TiO <subscript>2</subscript> NPs were internalized at various degrees and their toxicity depended on both titanium content and NPs shape, which impacted on intracellular calcium homeostasis thereby leading to endoplasmic reticulum stress. Finally, we showed that a minimal intracellular content of TiO <subscript>2</subscript> NPs was mandatory to induce toxicity enlightening once more the crucial notion of internalized dose threshold beside the well-recognized dose of exposure.
- Subjects :
- Animals
Cell Culture Techniques
Cell Proliferation drug effects
Endoplasmic Reticulum Stress genetics
HeLa Cells
Human Umbilical Vein Endothelial Cells
Humans
Keratinocytes
Particle Size
Real-Time Polymerase Chain Reaction
Surface Properties
Transcriptome drug effects
Endoplasmic Reticulum Stress drug effects
Nanoparticles analysis
Nanoparticles toxicity
Titanium analysis
Titanium toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1743-5404
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nanotoxicology
- Publication Type :
- Academic Journal
- Accession number :
- 28044465
- Full Text :
- https://doi.org/10.1080/17435390.2017.1278803