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Nervous necrosis virus capsid protein exploits nucleolar phosphoprotein Nucleophosmin (B23) function for viral replication.
- Source :
-
Virus research [Virus Res] 2017 Feb 15; Vol. 230, pp. 1-6. Date of Electronic Publication: 2016 Dec 26. - Publication Year :
- 2017
-
Abstract
- Nucleolar proteins facilitate the replication of certain human and animal viruses through interaction with viral proteins. In this study, an interaction between nervous necrosis virus capsid protein and nucleolar phosphoprotein B23 was identified using in vitro experimental approaches. The capsid protein binds to B23 early during the viral infection and accumulates in the nucleus, particularly in the nucleolus. However, over the course of the infection B23 is redistributed from the nucleoli to the nucleoplasm. siRNA-mediated knockdown of B23 reduced viral replication and cytopathic effect. Thus, B23 targets capsid protein to the nucleus and facilitates NNV replication. The results provide the first demonstration that nucleolar protein B23 has a direct role in the nodavirus replication process.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Capsid Proteins metabolism
Cell Nucleolus metabolism
Cell Nucleolus ultrastructure
Cell Nucleolus virology
Cloning, Molecular
Escherichia coli genetics
Escherichia coli metabolism
Fish Proteins antagonists & inhibitors
Fish Proteins metabolism
Gene Expression
Gene Expression Regulation
Genetic Vectors chemistry
Genetic Vectors metabolism
Host-Pathogen Interactions
Nodaviridae metabolism
Nuclear Proteins antagonists & inhibitors
Nuclear Proteins metabolism
Nucleophosmin
Perciformes virology
Phosphoproteins antagonists & inhibitors
Phosphoproteins metabolism
Protein Binding
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Capsid Proteins genetics
Fish Proteins genetics
Nodaviridae genetics
Nuclear Proteins genetics
Phosphoproteins genetics
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7492
- Volume :
- 230
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 28034778
- Full Text :
- https://doi.org/10.1016/j.virusres.2016.12.015