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Biomimetic calcium phosphate/polyelectrolyte multilayer coatings for sequential delivery of multiple biological factors.
- Source :
-
Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2017 May; Vol. 105 (5), pp. 1500-1509. Date of Electronic Publication: 2017 Mar 20. - Publication Year :
- 2017
-
Abstract
- Combinations of growth factors synergistically enhance tissue regeneration, but typically require sequential, rather than co-delivery from biomaterials for maximum efficacy. Polyelectrolyte multilayer (PEM) coatings can deliver multiple factors without loss of activity; however, sequential delivery from PEM has been limited due to interlayer diffusion that results in co-delivery of the factors. This study shows that addition of a biomimetic calcium phosphate (bCaP) barrier layer to a PEM coating effectively prevents interlayer diffusion and enables sequential delivery of two different biomolecules via direct cell access. A simulated body fluid method was used to deposit a layer of bCaP followed by 30 bilayers of PEM made with poly-l-Lysine (+) and poly l-Glutamic acid (-) (bCaP-PEM). Measurements of MC3T3-E1 proliferation and viability over time on bCaP-PEM were used to demonstrate the sequential delivery kinetics of a proliferative factor [fibroblast growth factor-2 (FGF-2)] followed by a cytotoxic factor (antimycin A, AntiA). FGF-2 and AntiA both retained their bioactivity within bCaP-PEM, yet no release of FGF-2 or AntiA from bCaP-PEM was observed when cells were absent indicating a cell-mediated, local delivery process. This coating technique is useful for a variety of applications that would benefit from highly localized, sequential delivery of multiple biomolecules governed by cell initiated degradation that avoids off-target effects associated with diffusion-based release. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1500-1509, 2017.<br /> (© 2017 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Cell Line
Cell Proliferation drug effects
Mice
Antimycin A chemistry
Antimycin A pharmacokinetics
Antimycin A pharmacology
Biomimetic Materials chemistry
Biomimetic Materials pharmacokinetics
Biomimetic Materials pharmacology
Calcium Phosphates chemistry
Calcium Phosphates pharmacokinetics
Calcium Phosphates pharmacology
Coated Materials, Biocompatible chemistry
Coated Materials, Biocompatible pharmacokinetics
Coated Materials, Biocompatible pharmacology
Drug Delivery Systems methods
Fibroblast Growth Factor 2 chemistry
Fibroblast Growth Factor 2 pharmacokinetics
Fibroblast Growth Factor 2 pharmacology
Polyelectrolytes chemistry
Polyelectrolytes pharmacokinetics
Polyelectrolytes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1552-4965
- Volume :
- 105
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of biomedical materials research. Part A
- Publication Type :
- Academic Journal
- Accession number :
- 28002652
- Full Text :
- https://doi.org/10.1002/jbm.a.35985