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Acute and chronic hyperglycemic effects of vasopressin in normal rats: involvement of V 1A receptors.
- Source :
-
American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2017 Mar 01; Vol. 312 (3), pp. E127-E135. Date of Electronic Publication: 2016 Dec 20. - Publication Year :
- 2017
-
Abstract
- Recent epidemiological studies have revealed novel relationships between low water intake or high vasopressin (AVP) and the risk of hyperglycemia and diabetes. AVP V <subscript>1A</subscript> and V <subscript>1B</subscript> receptors (R) are expressed in the liver and pancreatic islets, respectively. The present study was designed to determine the impact of different levels of circulating AVP on glucose homeostasis in normal Sprague-Dawley rats, as well as the respective roles of V <subscript>1A</subscript> R and V <subscript>1B</subscript> R. We showed that acute injection of AVP induces a dose-dependent increase in glycemia. Pretreatment with a selective V <subscript>1A</subscript> R antagonist, but not a V <subscript>1B</subscript> R antagonist, dose-dependently prevented the rise in glycemia. V <subscript>1B</subscript> R antagonism did not modify the hyperinsulinemic response, resulting from AVP-induced hyperglycemia, but enhanced the fall in glucagonemia. Acute administration of selective V <subscript>1A</subscript> R or V <subscript>1B</subscript> R agonists confirmed the involvement of V <subscript>1A</subscript> R in the hyperglycemic effect of AVP. In chronic experiments, AVP levels were altered in both directions. Sustained AVP infusion through implantable minipumps induced a time-dependent increase in fasting glycemia, whereas lowering endogenous AVP by increasing water intake had no effect. After 4 wk of AVP infusion, the rise in glycemia amounted to 1.1 mmol/l ( P < 0.01) without significant change in insulinemia. This effect was attenuated by cotreatment with a V <subscript>1A</subscript> R antagonist. Similar results were observed in lean Zucker rats. These findings demonstrate for the first time a causal link between chronic high AVP and hyperglycemia through V <subscript>1A</subscript> R activation and, thus, provide a pathophysiological explanation for the relationship observed in human cohorts between the AVP-hydration axis and the risk of diabetes.<br /> (Copyright © 2017 the American Physiological Society.)
- Subjects :
- Animals
Antidiuretic Hormone Receptor Antagonists pharmacology
Blood Glucose metabolism
Gene Knock-In Techniques
Glucagon blood
Hyperinsulinism blood
Indoles pharmacology
Insulin blood
Male
Optical Imaging
Pancreas metabolism
Peptides, Cyclic pharmacology
Pyrrolidines pharmacology
Rats
Rats, Sprague-Dawley
Rats, Zucker
Receptors, Vasopressin agonists
Receptors, Vasopressin metabolism
Arginine Vasopressin pharmacology
Blood Glucose drug effects
Glucagon drug effects
Hyperglycemia blood
Receptors, Vasopressin drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1555
- Volume :
- 312
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Endocrinology and metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 27998960
- Full Text :
- https://doi.org/10.1152/ajpendo.00269.2016