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Borrelia miyamotoi and Co-Infection with Borrelia afzelii in Ixodes ricinus Ticks and Rodents from Slovakia.
- Source :
-
Microbial ecology [Microb Ecol] 2017 May; Vol. 73 (4), pp. 1000-1008. Date of Electronic Publication: 2016 Dec 19. - Publication Year :
- 2017
-
Abstract
- Borrelia miyamotoi causes relapsing fever in humans. The occurrence of this spirochete has been reported in Ixodes ricinus and wildlife, but there are still gaps in the knowledge of its eco-epidemiology and public health impact. In the current study, questing I. ricinus (nymphs and adults) and skin biopsies from rodents captured in Slovakia were screened for the presence of B. miyamotoi and Borrelia burgdorferi s.l. DNA. The prevalence of B. miyamotoi and B. burgdorferi s.l. in questing ticks was 1.7 and 16.9%, respectively. B. miyamotoi was detected in Apodemus flavicollis (9.3%) and Myodes glareolus (4.4%). In contrast, B. burgdorferi s.l. was identified in 11.9% of rodents, with the highest prevalence in Microtus arvalis (68.4%) and a lower prevalence in Apodemus spp. (8.4%) and M. glareolus (12.4%). Borrelia afzelii was the prevailing genospecies infecting questing I. ricinus (37.9%) and rodents (72.2%). Co-infections of B. miyamotoi and B. burgdorferi s.l. were found in 24.1 and 9.3% of the questing ticks and rodents, respectively, whereas the proportion of ticks and rodents co-infected with B. miyamotoi and B. afzelii was 6.9 and 7.0%, respectively. The results suggest that B. miyamotoi and B. afzelii share amplifying hosts. The sequences of the B. miyamotoi glpQ gene fragment from our study showed a high degree of identity with sequences of the gene amplified from ticks and human patients in Europe. The results seem to suggest that humans in Slovakia are at risk of contracting tick-borne relapsing fever, and in some cases together with Lyme borreliosis.
- Subjects :
- Animals
Arvicolinae microbiology
Arvicolinae parasitology
Bacterial Proteins genetics
Borrelia genetics
Borrelia burgdorferi Group genetics
DNA, Bacterial chemistry
Disease Reservoirs microbiology
Disease Reservoirs veterinary
Europe
Female
Genes, Bacterial
Insect Vectors microbiology
Lyme Disease epidemiology
Male
Murinae microbiology
Murinae parasitology
Nymph microbiology
Phosphoric Diester Hydrolases genetics
Phylogeny
Prevalence
Rodent Diseases epidemiology
Rodent Diseases microbiology
Rodentia parasitology
Sequence Analysis
Slovakia epidemiology
Borrelia isolation & purification
Borrelia burgdorferi Group isolation & purification
Coinfection
Ixodes microbiology
Lyme Disease microbiology
Lyme Disease veterinary
Rodentia microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-184X
- Volume :
- 73
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microbial ecology
- Publication Type :
- Academic Journal
- Accession number :
- 27995301
- Full Text :
- https://doi.org/10.1007/s00248-016-0918-2