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Comparative analysis of cadmium responsive microRNAs in roots of two Ipomoea aquatica Forsk. cultivars with different cadmium accumulation capacities.
- Source :
-
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2017 Feb; Vol. 111, pp. 329-339. Date of Electronic Publication: 2016 Dec 12. - Publication Year :
- 2017
-
Abstract
- In plants, microRNAs (miRNAs) play regulatory roles in response to various environmental stresses. In order to illustrate the regulation mechanisms of miRNAs involving the different Cd accumulation abilities between a low-shoot-Cd cultivar (QLQ) and a high-shoot-Cd cultivar (T308) of water spinach (Ipomoea aquatic Forsk.), six sRNA libraries at 3 different time points were constructed. Only 5 miRNAs were exclusively regulated in QLQ, among them, miRNA395 was up-regulated, which was supposed to enhance the Cd retention and detoxification in root. Also, the alterations of miRNA5139, miRNA1511 and miRNA8155 contributed to the attenuation of Cd translocation into the shoot of QLQ. More differentially expressed miRNAs were observed in T308, indicating more complex response was adopted by T308 under Cd stress. miRNA397 exclusively regulated in T308 has enhanced the Cd influx of T308 under Cd treatments. Besides, the Cd translocation of T308 was strengthened due to the up-regulation of MATE efflux family, which was targeted by miRNA3627. Our results unraveled the effects of the cultivar-dependent expression of these specific miRNAs on the different Cd accumulation and translocation abilities of QLQ and T308. These findings provide a new perspective for the molecular assisted breeding of low-Cd cultivars for leaf-vegetables.<br /> (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Cluster Analysis
Gene Expression Regulation, Plant drug effects
Gene Ontology
Ipomoea drug effects
MicroRNAs metabolism
Plant Roots drug effects
Plant Shoots drug effects
Plant Shoots metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
RNA, Plant genetics
RNA, Plant metabolism
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Sequence Analysis, RNA
Spinacia oleracea metabolism
Water metabolism
Cadmium toxicity
Ipomoea genetics
MicroRNAs genetics
Plant Roots genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2690
- Volume :
- 111
- Database :
- MEDLINE
- Journal :
- Plant physiology and biochemistry : PPB
- Publication Type :
- Academic Journal
- Accession number :
- 27992771
- Full Text :
- https://doi.org/10.1016/j.plaphy.2016.12.013