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Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.
- Source :
-
Nature neuroscience [Nat Neurosci] 2017 Feb; Vol. 20 (2), pp. 176-188. Date of Electronic Publication: 2016 Dec 19. - Publication Year :
- 2017
-
Abstract
- The hypothalamus contains the highest diversity of neurons in the brain. Many of these neurons can co-release neurotransmitters and neuropeptides in a use-dependent manner. Investigators have hitherto relied on candidate protein-based tools to correlate behavioral, endocrine and gender traits with hypothalamic neuron identity. Here we map neuronal identities in the hypothalamus by single-cell RNA sequencing. We distinguished 62 neuronal subtypes producing glutamatergic, dopaminergic or GABAergic markers for synaptic neurotransmission and harboring the ability to engage in task-dependent neurotransmitter switching. We identified dopamine neurons that uniquely coexpress the Onecut3 and Nmur2 genes, and placed these in the periventricular nucleus with many synaptic afferents arising from neuromedin S <superscript>+</superscript> neurons of the suprachiasmatic nucleus. These neuroendocrine dopamine cells may contribute to the dopaminergic inhibition of prolactin secretion diurnally, as their neuromedin S <superscript>+</superscript> inputs originate from neurons expressing Per2 and Per3 and their tyrosine hydroxylase phosphorylation is regulated in a circadian fashion. Overall, our catalog of neuronal subclasses provides new understanding of hypothalamic organization and function.
- Subjects :
- Animals
Immunohistochemistry methods
Mice, Inbred C57BL
Mice, Transgenic
Neurotransmitter Agents physiology
Suprachiasmatic Nucleus metabolism
Synaptic Transmission physiology
Dopamine metabolism
Dopaminergic Neurons metabolism
Hypothalamus metabolism
Neuropeptides metabolism
Tyrosine 3-Monooxygenase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1546-1726
- Volume :
- 20
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nature neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 27991900
- Full Text :
- https://doi.org/10.1038/nn.4462