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Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2016 Nov 28; Vol. 22 (44), pp. 9844-9852. - Publication Year :
- 2016
-
Abstract
- Aim: To assess disease-specific circulating microRNAs (miRNAs) in non-alcoholic steatohepatitis (NASH) patients.<br />Methods: A total of 111 biopsy-proven non-alcoholic fatty liver disease (NAFLD) or chronic hepatitis B (CHB) patients and healthy controls from mainland China were enrolled to measure their serum levels of miR-122, -125b, -146b, -16, -21, -192, -27b and -34a. The correlations between serum miRNAs and histological features of NAFLD were determined. The diagnostic value of miRNA in NASH and significant fibrosis was analyzed and compared with that of cytokeratin-18 (CK-18), fibrosis-4 (FIB-4), and aspartate aminotransferase to platelet ratio index (APRI), respectively.<br />Results: Circulating miR-122, -16, -192 and -34a showed differential expression levels between NAFLD and CHB patients, and miR-34a had an approximately 2-fold increase in NAFLD samples compared with that of CHB samples ( P < 0.01). Serum miR-122, -192 and -34a levels were correlated with steatosis (R = 0.302, 0.323 and 0.470, respectively, P < 0.05) and inflammatory activity (R = 0.445, 0.447 and 0.517, respectively, P < 0.01); only serum miR-16 levels were associated with fibrosis (R = 0.350, P < 0.05) in patients with NAFLD. The diagnostic value of miR-34a for NASH (area under the receiver operating characteristic, 0.811, 95%CI: 0.670-0.953) was superior to that of alanine aminotransferase, CK-18, FIB-4 and APRI in NAFLD, but miR-16 showed a limited performance in the diagnosis of significant fibrosis in NASH.<br />Conclusion: Circulating miR-34a may serve as a disease-specific noninvasive biomarker for the diagnosis of NASH.<br />Competing Interests: Conflict-of-interest statement: The authors declare that they have no conflict of interest.
- Subjects :
- Adult
Alanine Transaminase blood
Area Under Curve
Asian People genetics
Aspartate Aminotransferases blood
Biopsy
Case-Control Studies
China epidemiology
Female
Genetic Markers
Hepatitis B, Chronic blood
Hepatitis B, Chronic diagnosis
Hepatitis B, Chronic ethnology
Humans
Keratin-18 blood
Liver Cirrhosis blood
Liver Cirrhosis diagnosis
Liver Cirrhosis ethnology
Liver Cirrhosis genetics
Male
MicroRNAs blood
Middle Aged
Non-alcoholic Fatty Liver Disease blood
Non-alcoholic Fatty Liver Disease diagnosis
Non-alcoholic Fatty Liver Disease ethnology
Platelet Count
Predictive Value of Tests
ROC Curve
Hepatitis B, Chronic genetics
MicroRNAs genetics
Non-alcoholic Fatty Liver Disease genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 22
- Issue :
- 44
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 27956809
- Full Text :
- https://doi.org/10.3748/wjg.v22.i44.9844