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The chemokinome superfamily in channel catfish: I. CXC subfamily and their involvement in disease defense and hypoxia responses.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2017 Jan; Vol. 60, pp. 380-390. Date of Electronic Publication: 2016 Dec 03. - Publication Year :
- 2017
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Abstract
- Chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. They are defined by the presence of four conserved cysteine residues and are divided into four subfamilies depending on the arrangement of the first two conserved cysteines residues: CXC, CC, C and CX3C. In this study, a complete set of 17 CXC chemokine ligand (CXCL) genes was systematically identified and characterized from channel catfish genome through data mining of existing genomic resources. Phylogenetic analysis allowed annotation of the 17 CXC chemokines. Extensive comparative genomic analyses supported their annotations and orthologies, revealing the existence of fish-specific CXC chemokines and the expansion of CXC chemokines in the teleost genomes. The analysis of gene expression after bacterial infection indicated the CXC chemokines were expressed in a gene-specific manner. CXCL11.3 and CXCL20.3 were expressed significantly higher in resistant fish than in susceptible fish after ESC infection, while CXCL20.2 were expressed significantly higher in resistant fish than in susceptible fish after columnaris infection. The expression of those CXC chemokines, therefore can be a useful indicator of disease resistance. A similar pattern of expression was observed between resistant and susceptible fish with biotic and abiotic stresses, ESC, columnaris and hypoxia, suggesting that high levels of expression of the majority of CXC chemokines, with exception of CXC11 and CXC20, are detrimental to the host.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Anaerobiosis genetics
Animals
Chemokines, CXC chemistry
Chemokines, CXC metabolism
Disease Resistance genetics
Edwardsiella ictaluri physiology
Enterobacteriaceae Infections genetics
Enterobacteriaceae Infections immunology
Enterobacteriaceae Infections microbiology
Fish Diseases immunology
Fish Diseases microbiology
Fish Proteins chemistry
Fish Proteins metabolism
Flavobacteriaceae Infections genetics
Flavobacteriaceae Infections immunology
Flavobacteriaceae Infections microbiology
Flavobacterium physiology
Phylogeny
Sequence Analysis, DNA veterinary
Synteny
Chemokines, CXC genetics
Enterobacteriaceae Infections veterinary
Fish Diseases genetics
Fish Proteins genetics
Flavobacteriaceae Infections veterinary
Gene Expression
Ictaluridae
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 60
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 27919758
- Full Text :
- https://doi.org/10.1016/j.fsi.2016.12.004