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Cyclosporine A inhibits TGF-β2-induced myofibroblasts of primary cultured human pterygium fibroblasts.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2017 Jan 22; Vol. 482 (4), pp. 1148-1153. Date of Electronic Publication: 2016 Dec 02. - Publication Year :
- 2017
-
Abstract
- Cyclosporine A (CsA), an immunomodulatory drug, and is increasingly used to treat moderate dry eye syndrome and ocular surface inflammation. However, any inhibitory effect on differentiation of fibroblasts to myofibroblasts remains unclear. Here, we show that the inhibitory effect of CsA on transforming growth factor-beta2 (TGF-β2)-induced myofibroblasts in primary cultured human pterygium fibroblasts. CsA significantly decreased mRNA and protein expression of myofibroblast-related markers including α-SMA, laminin, and fibronectin. These findings were supported by the results from immunofluorescence staining. Taken together, these results indicate the therapeutic potential of CsA against pterygium progression. Further studies are necessary to elucidate the precise intracellular signal mechanism responsible for CsA-induced downregulation of myofibroblast markers in pterygium fibroblasts.<br /> (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Actins metabolism
Cell Differentiation
Cells, Cultured
Female
Fibronectins metabolism
Humans
Immunosuppressive Agents pharmacology
Inflammation
Laminin metabolism
Male
Microscopy, Fluorescence
Muscle, Smooth metabolism
Myofibroblasts metabolism
Oligonucleotides chemistry
Pterygium surgery
Signal Transduction
Software
Transforming Growth Factor beta2 pharmacology
Cyclosporine pharmacology
Fibroblasts metabolism
Pterygium drug therapy
Pterygium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 482
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 27919682
- Full Text :
- https://doi.org/10.1016/j.bbrc.2016.12.002