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Effect of dimethyl sulfoxide and protein concentration on the viability of two-cell mouse embryos frozen with a rapid freezing technique.
- Source :
-
Cryobiology [Cryobiology] 1989 Oct; Vol. 26 (5), pp. 413-21. - Publication Year :
- 1989
-
Abstract
- Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged during freezing and thawing. Increasing the BSA concentration from 4 to 32 mg/ml had no significant effect on subsequent embryo viability in vivo or in vitro. Blastocyst formation in vitro was greater than 90% in embryos exposed to the cryoprotective solutions only. Although development to blastocysts was not significantly different to nonfrozen controls in most groups frozen in 3 and 4.5 M Me2SO (up to 92% blastocysts), it was significantly reduced when embryos were frozen in 1.5 M Me2SO (up to 65% blastocysts). The development to fetuses of embryos frozen in 3 M Me2SO (64 to 74% fetuses) was not significantly different from nonfrozen controls (68 to 79% fetuses) or embryos frozen by a conventional slow cooling method (70%). Frozen thawed two-cell embryos developed into normal adults which were able to reproduce normally. We conclude that this freezing method can efficiently cryopreserve early cleavage stage mouse embryos.
- Subjects :
- Animals
Blastocyst drug effects
Congenital Abnormalities
Dimethyl Sulfoxide administration & dosage
Embryo Transfer
Embryonic and Fetal Development
Female
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Serum Albumin, Bovine administration & dosage
Blastocyst physiology
Cryopreservation
Dimethyl Sulfoxide pharmacology
Serum Albumin, Bovine pharmacology
Tissue Preservation
Subjects
Details
- Language :
- English
- ISSN :
- 0011-2240
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cryobiology
- Publication Type :
- Academic Journal
- Accession number :
- 2791610
- Full Text :
- https://doi.org/10.1016/0011-2240(89)90066-7