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[Isolation of monoclonal antibodies from mouse ascitic fluid using chromatography on "blue" DEAE cellulose].

Authors :
Hrkal Z
Rejnková J
Stöckbauer P
Rybák M
Benes MJ
Source :
Ceskoslovenska farmacie [Cesk Farm] 1989 Jul; Vol. 38 (6), pp. 276-8.
Publication Year :
1989

Abstract

A method is described of the isolation of monoclonal antibodies form mouse ascites fluids by chromatography on beaded DEAE cellulose with covalently bound Reactive Blue-2 ("Blue" DEAE cellulose). The method yields antibodies of 80-90% purity, free from protease activity.

Details

Language :
Czech
ISSN :
0009-0530
Volume :
38
Issue :
6
Database :
MEDLINE
Journal :
Ceskoslovenska farmacie
Publication Type :
Academic Journal
Accession number :
2790996