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Streptococcus himalayensis sp. nov., isolated from the respiratory tract of Marmota himalayana.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2017 Feb; Vol. 67 (2), pp. 256-261. Date of Electronic Publication: 2017 Feb 21. - Publication Year :
- 2017
-
Abstract
- Five strains of Gram-positive-staining, catalase-negative, coccus-shaped, chain-forming organisms isolated separately from the respiratory tracts of five Marmota himalayana animals in the Qinghai-Tibet Plateau of China were subjected to phenotypic and molecular taxonomic analyses. Comparative analysis of the 16S rRNA gene indicated that these singular organisms represent a new member of the genus Streptococcus, being phylogenetically closest to Streptococcus marmotae DSM 101995T (98.4 % similarity). The groEL, sodA and rpoB sequence analysis showed interspecies similarity values between HTS2T and Streptococcus. marmotae DSM 101995T, its closest phylogenetic relative based on 16S rRNA gene sequences, of 98.2, 78.8 and 93.7 %, respectively. A whole-genome phylogenetic tree built from 82 core genes of genomes from 16 species of the genus Streptococcus validated that HTS2T forms a distinct subline and exhibits specific phylogenetic affinity with S. marmotae. In silico DNA-DNA hybridization of HTS2T showed an estimated DNA reassociation value of 40.5 % with Streptococcus. marmotae DSM 101995T. On the basis of their phenotypic characteristics and phylogenetic findings, it is proposed that the five isolates be classified as representatives of a novel species of the genus Streptococcus, Streptococcus himalayensis sp. nov. The type strain is HTS2T (=DSM 101997T=CGMCC 1.15533T). The genome of Streptococcus himalayensis sp. nov. strain HTS2T contains 2195 genes with a size of 2 275 471 bp and a mean DNA G+C content of 41.3 mol%.
- Subjects :
- Animals
Bacterial Typing Techniques
Base Composition
DNA, Bacterial genetics
Nucleic Acid Hybridization
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Streptococcus genetics
Streptococcus isolation & purification
Tibet
Marmota microbiology
Phylogeny
Respiratory System microbiology
Streptococcus classification
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 67
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 27902227
- Full Text :
- https://doi.org/10.1099/ijsem.0.001609