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Ouabain induces apoptosis and autophagy in Burkitt's lymphoma Raji cells.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2016 Dec; Vol. 84, pp. 1841-1848. Date of Electronic Publication: 2016 Nov 25. - Publication Year :
- 2016
-
Abstract
- The steroid Na+/K+-ATPase blocker ouabain has been shown to exhibit cytotoxic effects in various tumor cell systems. This study aimed to determine the effects of ouabain on Burkitt's lymphoma Raji cells. Ouabain treatment of Raji cells significantly inhibited cell proliferation in a dose-dependent manner and increased the morphological changes associated with apoptosis. Additionally, increased numbers of both early and late apoptotic cells were observed by annexin V-FITC/PI flow cytometry assay. Increased levels of caspase-3 and cleaved-caspase-3, higher Bax activity and decreased expression of the anti-apoptotic protein Bcl-2 were detected in ouabain-treated Raji cells. Vacuole accumulation was also observed in transmission electron microscope (TEM) images of ouabain-treated Raji cells, indicating that these cells were undergoing autophagy. Expression of the autophagy-related proteins LC3-II and Beclin-1 was upregulated in ouabain-treated Raji cells. These results suggest that ouabain may promote cell death in Raji cells by inducing pathways associated with apoptosis and autophagy. Our study also provides novel evidence that ouabain may be an effective agent for treating Burkitt's lymphoma.<br /> (Copyright © 2016. Published by Elsevier Masson SAS.)
- Subjects :
- Apoptosis Regulatory Proteins metabolism
Beclin-1 metabolism
Burkitt Lymphoma metabolism
Burkitt Lymphoma pathology
Cell Line, Tumor
Cell Proliferation drug effects
Dose-Response Relationship, Drug
Humans
Microtubule-Associated Proteins metabolism
Signal Transduction drug effects
Time Factors
Antineoplastic Agents pharmacology
Apoptosis drug effects
Autophagy drug effects
Burkitt Lymphoma drug therapy
Ouabain pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 84
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 27894666
- Full Text :
- https://doi.org/10.1016/j.biopha.2016.10.114