Back to Search
Start Over
The identification of human pituitary adenoma-initiating cells.
- Source :
-
Acta neuropathologica communications [Acta Neuropathol Commun] 2016 Nov 28; Vol. 4 (1), pp. 125. Date of Electronic Publication: 2016 Nov 28. - Publication Year :
- 2016
-
Abstract
- Classified as benign central nervous system (CNS) tumors, pituitary adenomas account for 10% of diagnosed intracranial neoplasms. Although surgery is often curative, patients with invasive macroadenomas continue to experience significant morbidity and are prone to tumor recurrence. Given the identification of human brain tumor-initiating cells (TICs) that initiate and maintain tumor growth while promoting disease progression and relapse in multiple CNS tumors, we investigated whether TICs also drive the growth of human pituitary adenomas. Using a nanoString-based 80-gene custom codeset specific for developmental pathways, we identified a differential stem cell gene expression profile within human pituitary adenomas. Prospective functional characterization of stem cell properties in patient-derived adenomas representing all hormonal subtypes yielded a subtype-dependent self-renewal profile, which was enriched within the CD15+ cell fraction. The tumor-initiating capacity of CD15 <superscript>high</superscript> adenoma cells was assayed in comparison to CD15 <superscript>low</superscript> adenomas using in vivo limiting dilutions, which maintained the rare frequency of TICs. Repeated analyses using sorted cell populations for CD15+ TICs compared to CD15- adenoma cells provided further evidence of xenograft tumor formation to support CD15+ cells as putative pituitary adenoma-initiating cells (PAICs). The clinical utility of our findings was established through in silico analyses and comparative gene expression profiling of primary and recurrent pituitary adenomas. CD15 was enriched in recurrent adenomas, which was validated using routine clinical immunohistochemistry in a limited number of samples. Our work reports the first prospective identification of human PAICs using CD15. Patients with CD15 <superscript>high</superscript> adenomas may therefore benefit from more aggressive surgical interventions and chemo/radiotherapy.
- Subjects :
- Adenoma genetics
Adult
Aged
Aged, 80 and over
Animals
Biomarkers, Tumor genetics
Biomarkers, Tumor metabolism
Female
Flow Cytometry
Gene Expression Profiling
Humans
Immunohistochemistry
Male
Mice, Inbred NOD
Mice, SCID
Middle Aged
Neoplasm Recurrence, Local genetics
Neoplasm Recurrence, Local metabolism
Neoplasm Transplantation
Pituitary Neoplasms genetics
Real-Time Polymerase Chain Reaction
Single-Cell Analysis
Adenoma metabolism
Brain metabolism
Lewis X Antigen metabolism
Neoplastic Stem Cells metabolism
Pituitary Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2051-5960
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Acta neuropathologica communications
- Publication Type :
- Academic Journal
- Accession number :
- 27894339
- Full Text :
- https://doi.org/10.1186/s40478-016-0394-4