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Use of Fe₃O₄ Nanoparticles for Enhancement of Biosensor Response to the Herbicide 2,4-Dichlorophenoxyacetic Acid.
- Source :
-
Sensors (Basel, Switzerland) [Sensors (Basel)] 2008 Sep 18; Vol. 8 (9), pp. 5775-5791. Date of Electronic Publication: 2008 Sep 18. - Publication Year :
- 2008
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Abstract
- Magnetic nanoparticles of Fe₃O₄ were synthesized and characterized using transmission electron microscopy and X-ray diffraction. The Fe₃O₄ nanoparticles were found to have an average diameter of 5.48 ±1.37 nm. An electrochemical biosensor based on immobilized alkaline phosphatase (ALP) and Fe₃O₄ nanoparticles was studied. The amperometric biosensor was based on the reaction of ALP with the substrate ascorbic acid 2-phosphate (AA2P). The incorporation of the Fe₃O₄ nanoparticles together with ALP into a sol gel/chitosan biosensor membrane has led to the enhancement of the biosensor response, with an improved linear response range to the substrate AA2P (5-120 μM) and increased sensitivity. Using the inhibition property of the ALP, the biosensor was applied to the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The use of Fe₃O₄ nanoparticles gives a two-fold improvement in the sensitivity towards 2,4-D, with a linear response range of 0.5-30 μgL <superscript>-1</superscript> . Exposure of the biosensor to other toxicants such as heavy metals demonstrated only slight interference from metals such as Hg <superscript>2+</superscript> , Cu <superscript>2+</superscript> , Ag <superscript>2+</superscript> and Pb <superscript>2+</superscript> . The biosensor was shown to be useful for the determination of the herbicide 2, 4-D because good recovery of 95-100 percent was obtained, even though the analysis was performed in water samples with a complex matrix. Furthermore, the results from the analysis of 2,4-D in water samples using the biosensor correlated well with a HPLC method.
Details
- Language :
- English
- ISSN :
- 1424-8220
- Volume :
- 8
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Sensors (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 27873839
- Full Text :
- https://doi.org/10.3390/s8095775