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Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes.

Authors :
Abdelfattah AS
Rancic V
Rawal B
Ballanyi K
Campbell RE
Source :
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2016 Dec 01; Vol. 52 (98), pp. 14153-14156.
Publication Year :
2016

Abstract

To expand the toolbox of fluorescent protein-based voltage indicators, we explored two distinct protein design strategies. Using these design strategies, we created three new voltage indicators: a red intensiometric voltage indicator (tdFlicR Δ110AR), a green/red ratiometric voltage indicator (tdFlicR-VK-ASAP), and a green to red photoconvertible voltage indicator (FlicGR1).

Details

Language :
English
ISSN :
1364-548X
Volume :
52
Issue :
98
Database :
MEDLINE
Journal :
Chemical communications (Cambridge, England)
Publication Type :
Academic Journal
Accession number :
27869275
Full Text :
https://doi.org/10.1039/c6cc06810c