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Heterogeneous nuclear ribonucleoprotein K is associated with poor prognosis and regulates proliferation and apoptosis in bladder cancer.
- Source :
-
Journal of cellular and molecular medicine [J Cell Mol Med] 2017 Jul; Vol. 21 (7), pp. 1266-1279. Date of Electronic Publication: 2016 Nov 10. - Publication Year :
- 2017
-
Abstract
- Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is an essential RNA- and DNA-binding protein that regulates diverse biological events, especially DNA transcription. hnRNPK overexpression is related to tumorigenesis in several cancers. However, both the expression patterns and biological mechanisms of hnRNPK in bladder cancer are unclear. We investigated hnRNPK expression by immunohistochemistry in 188 patients with bladder cancer, and found that hnRNPK expression levels were significantly increased in bladder cancer tissues and that high-hnRNPK expression was closely correlated with poor prognosis. Loss- and gain-of-function assays demonstrated that hnRNPK promoted proliferation, anti-apoptosis, and chemoresistance in bladder cancer cells in vitro, and hnRNPK knockdown suppressed tumorigenicity in vivo. Mechanistically, hnRNPK regulated various functions in bladder cancer by directly mediating cyclin D1, G0/G1 switch 2 (G0S2), XIAP-associated factor 1, and ERCC excision repair 4, endonuclease catalytic subunit (ERCC4) transcription. In conclusion, we discovered that hnRNPK plays an important role in bladder cancer, suggesting that it is a potential prognostic marker and a promising target for treating bladder cancer.<br /> (© 2016 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)
- Subjects :
- Aged
Apoptosis genetics
Cell Line, Tumor
DNA-Binding Proteins genetics
Disease-Free Survival
Female
Gene Expression Regulation, Neoplastic
Humans
Kaplan-Meier Estimate
Male
Middle Aged
Phosphorylation
Prognosis
Urinary Bladder Neoplasms pathology
Carcinogenesis genetics
Cell Proliferation genetics
Heterogeneous-Nuclear Ribonucleoprotein K genetics
Urinary Bladder Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1582-4934
- Volume :
- 21
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of cellular and molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 27862976
- Full Text :
- https://doi.org/10.1111/jcmm.12999