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Protein-Sized Bright Fluorogenic Nanoparticles Based on Cross-Linked Calixarene Micelles with Cyanine Corona.
- Source :
-
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2016 Dec 19; Vol. 55 (51), pp. 15884-15888. Date of Electronic Publication: 2016 Nov 16. - Publication Year :
- 2016
-
Abstract
- The key challenge in the field of fluorescent nanoparticles (NPs) for biological applications is to achieve superior brightness for sizes equivalent to single proteins (3-7 nm). We propose a concept of shell-cross-linked fluorescent micelles, in which PEGylated cyanine 3 and 5 bis-azides form a covalently attached corona on micelles of amphiphilic calixarene bearing four alkyne groups. The fluorescence quantum yield of the obtained monodisperse NPs, with a size of 7 nm, is a function of viscosity and reached up to 15 % in glycerol. In the on-state they are circa 2-fold brighter than quantum dots (QD-585), which makes them the smallest PEGylated organic NPs of this high brightness. FRET between cyanine 3 and 5 cross-linkers at the surface of NPs suggests their integrity in physiological media, organic solvents, and living cells, in which the NPs rapidly internalize, showing excellent imaging contrast. Calixarene micelles with a cyanine corona constitute a new platform for the development of protein-sized ultrabright fluorescent NPs.<br /> (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Azides chemistry
Click Chemistry
Cross-Linking Reagents chemistry
HeLa Cells
Humans
Nanoparticles ultrastructure
Optical Imaging methods
Particle Size
Polyethylene Glycols chemistry
Proteins chemistry
Calixarenes chemistry
Carbocyanines chemistry
Fluorescent Dyes chemistry
Micelles
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3773
- Volume :
- 55
- Issue :
- 51
- Database :
- MEDLINE
- Journal :
- Angewandte Chemie (International ed. in English)
- Publication Type :
- Academic Journal
- Accession number :
- 27862803
- Full Text :
- https://doi.org/10.1002/anie.201609138