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Human corneal fibroblast migration and extracellular matrix synthesis during stromal repair: Role played by platelet-derived growth factor-BB, basic fibroblast growth factor, and transforming growth factor-β1.
- Source :
-
Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2018 Feb; Vol. 12 (2), pp. e737-e746. Date of Electronic Publication: 2017 May 12. - Publication Year :
- 2018
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Abstract
- The development of treatments that modulate corneal wound healing to avoid fibrosis during tissue repair is important for the restoration of corneal transparency after an injury. To date, few studies have studied the influence of growth factors (GFs) on human corneal fibroblast (HCF) expression of extracellular matrix (ECM) proteins such as collagen types I and III, proteoglycans such as perlecan, or proteins implicated in cellular migration such as α5β1-integrin and syndecan-4. Using in vitro HCFs, a mechanical wound model was developed to study the influence of the GFs basic fibroblast GF (bFGF), platelet-derived GF (PDGF-BB) and transforming GF-β1 (TGFβ1) on ECM protein production and cellular migration. Our results show that mechanical wounding provokes the autocrine release of bFGF and TGFβ1 at different time points during the wound closure. The HCF response to PDGF-BB was a rapid closure due to fast cellular migration associated with a high focal adhesion replacement and a high expression of collagen and proteoglycans, producing nonfibrotic healing. bFGF stimulated nonfibrotic ECM production and limited the migration process. Finally, TGFβ1 induced expression of the fibrotic markers collagen type III and α5β1 integrin, and it inhibited cellular migration due to the formation of focal adhesions with a low turnover rate. The novel in vitro HCF mechanical wound model can be used to understand the role played by GFs in human corneal repair. The model can also be used to test the effects of different treatments aimed at improving the healing process. Copyright © 2016 John Wiley & Sons, Ltd.<br /> (Copyright © 2016 John Wiley & Sons, Ltd.)
- Subjects :
- Collagen Type I metabolism
Collagen Type III metabolism
Culture Media
Extracellular Matrix drug effects
Fibroblasts drug effects
Heparan Sulfate Proteoglycans genetics
Heparan Sulfate Proteoglycans metabolism
Humans
Integrins genetics
Integrins metabolism
Protein Subunits genetics
Protein Subunits metabolism
Proteoglycans genetics
Proteoglycans metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Stromal Cells drug effects
Stromal Cells pathology
Syndecan-4 genetics
Syndecan-4 metabolism
Wound Healing drug effects
Becaplermin pharmacology
Cell Movement drug effects
Cornea cytology
Extracellular Matrix metabolism
Fibroblast Growth Factor 2 pharmacology
Fibroblasts cytology
Transforming Growth Factor beta1 pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-7005
- Volume :
- 12
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of tissue engineering and regenerative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 27860426
- Full Text :
- https://doi.org/10.1002/term.2360