Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion.

Authors :: Cai W
Wei Q
Liu Q
Ren C
Liu J
Zhang R
He M
Wang Q
Du Y
Yu J
Source :: BMC ophthalmology [BMC Ophthalmol] 2016 Nov 10; Vol. 16 (1), pp. 199. Date of Electronic Publication: 2016 Nov 10.
Publication Year :: 2016
Abstract: Background: To evaluate the effect of bradykinin (BK) on TGF-β1-induced retinal pigment epithelial (RPE) cell proliferation and extracellular matrix secretion and to elucidate the relationship between BK and the Erk/Akt signaling pathway.<br />Methods: The effects of BK on TGF-β1-induced RPE cell proliferation were examined via CCK-8 assay. Cell culture supernatant collagen I concentrations were measured via ELISA. Fibronectin (Fn), matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA and protein expression levels were measured via q-PCR and Western blotting, respectively. Changes in Akt/Erk phosphorylation induced by BK and HOE-140 were evaluated via Western blotting.<br />Results: TGF-β1 stimulated ARPE-19 cell proliferation, which was inhibited by BK, whose effects were inhibited by HOE-140. BK inhibited TGF-β1-induced collagen I, Fn and MMP-2 secretion in RPE cells, and these effects were inhibited by HOE-140. BK also inhibited TGF-β1-induced Akt phosphorylation in RPE cells, and these effects were blocked by HOE-140. BK had no significant effect on Erk-mediated signaling.<br />Conclusions: The findings from this study indicate that BK could be novel therapeutic targets for the treatment of PVR.

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