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Cellulase from Trichoderma harzianum interacts with roots and triggers induced systemic resistance to foliar disease in maize.
- Source :
-
Scientific reports [Sci Rep] 2016 Nov 10; Vol. 6, pp. 35543. Date of Electronic Publication: 2016 Nov 10. - Publication Year :
- 2016
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Abstract
- Trichoderma harzianum is well known to exhibit induced systemic resistance (ISR) to Curvularia leaf spot. We previously reported that a C6 zinc finger protein (Thc6) is responsible for a major contribution to the ISR to the leaf disease, but the types of effectors and the signals mediated by Thc6 from Trichoderma are unclear. In this work, we demonstrated that two hydrolases, Thph1 and Thph2, from T. harzianum were regulated by Thc6. Furthermore, an electrophoretic mobility shift assay (EMSA) study revealed that Thc6 regulated mRNA expression by binding to GGCTAA and GGCTAAA in the promoters of the Thph1 and Thph2 genes, respectively. Moreover, the Thph1 and Thph2 proteins triggered the transient production of reactive oxygen species (ROS) and elevated the free cytosolic calcium levels in maize leaf. Furthermore, the genes related to the jasmonate/ethylene signaling pathway were up-regulated in the wild-type maize strain. However, the ΔThph1- or ΔThph2-deletion mutants could not activate the immune defense-related genes in maize to protect against leaf disease. Therefore, we conclude that functional Thph1 and Thph2 may be required in T. harzianum to activate ISR in maize.
- Subjects :
- Binding Sites
Calcium metabolism
Fungal Proteins chemistry
Fungal Proteins genetics
Fungal Proteins metabolism
Gene Expression Regulation, Plant
Hydrolases chemistry
Plant Diseases microbiology
Plant Proteins genetics
Plant Roots metabolism
Reactive Oxygen Species metabolism
Disease Resistance
Hydrolases genetics
Saccharomycetales pathogenicity
Transcription Factors metabolism
Trichoderma enzymology
Zea mays microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27830829
- Full Text :
- https://doi.org/10.1038/srep35543