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Senescence Phenotypes Induced by Ras in Primary Cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1534, pp. 17-30. - Publication Year :
- 2017
-
Abstract
- Cellular senescence is defined as a state of stable cell-cycle arrest that is distinct from quiescence and terminal differentiation. Many stimuli can induce senescence, including telomere shortening and oncogene activation. The phenotypes elicited by pro-senescent signals can be heterogeneous depending on the stimulus and the cell type affected. To date, there is not a definitive marker that can ubiquitously and specifically mark all senescent cells. Therefore, several independent markers must be utilized to ascertain the senescent state of a cell or group of cells. Here, we describe common assays used to assess oncogenic Ras-induced senescence.
- Subjects :
- Biomarkers
Cell Cycle genetics
Cell Line, Transformed
Cells, Cultured
Chromatin Assembly and Disassembly
Fibroblasts metabolism
Gene Expression Regulation
Heterochromatin genetics
Heterochromatin metabolism
Humans
Primary Cell Culture
Subtilisins genetics
Subtilisins metabolism
beta-Galactosidase metabolism
Cellular Senescence physiology
Oncogene Protein p21(ras) genetics
Oncogene Protein p21(ras) metabolism
Phenotype
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1534
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 27812864
- Full Text :
- https://doi.org/10.1007/978-1-4939-6670-7_2