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Biophysical Properties and Motility of Human Mature Dendritic Cells Deteriorated by Vascular Endothelial Growth Factor through Cytoskeleton Remodeling.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2016 Oct 31; Vol. 17 (11). Date of Electronic Publication: 2016 Oct 31. - Publication Year :
- 2016
-
Abstract
- Dendritic cells (DCs), the most potent antigen-presenting cells, play a central role in the initiation, regulation, and maintenance of the immune responses. Vascular endothelial growth factor (VEGF) is one of the important cytokines in the tumor microenvironment (TME) and can inhibit the differentiation and functional maturation of DCs. To elucidate the potential mechanisms of DC dysfunction induced by VEGF, the effects of VEGF on the biophysical characteristics and motility of human mature DCs (mDCs) were investigated. The results showed that VEGF had a negative influence on the biophysical properties, including electrophoretic mobility, osmotic fragility, viscoelasticity, and transmigration. Further cytoskeleton structure analysis by confocal microscope and gene expression profile analyses by gene microarray and real-time PCR indicated that the abnormal remodeling of F-actin cytoskeleton may be the main reason for the deterioration of biophysical properties, motility, and stimulatory capability of VEGF-treated mDCs. This is significant for understanding the biological behavior of DCs and the immune escape mechanism of tumors. Simultaneously, the therapeutic efficacies may be improved by blocking the signaling pathway of VEGF in an appropriate manner before the deployment of DC-based vaccinations against tumors.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Actins metabolism
Apoptosis drug effects
Cytoskeleton drug effects
Dendritic Cells drug effects
Down-Regulation drug effects
Elasticity
Electrophoresis
Gene Expression Profiling
Gene Ontology
Human Umbilical Vein Endothelial Cells drug effects
Human Umbilical Vein Endothelial Cells metabolism
Humans
Osmotic Fragility drug effects
Pseudopodia drug effects
Pseudopodia metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Transendothelial and Transepithelial Migration drug effects
Up-Regulation drug effects
Viscosity
Biophysical Phenomena drug effects
Cell Differentiation drug effects
Cell Movement drug effects
Cytoskeleton metabolism
Dendritic Cells cytology
Dendritic Cells metabolism
Vascular Endothelial Growth Factor A pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 17
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 27809226
- Full Text :
- https://doi.org/10.3390/ijms17111756