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Sirt1 expression is associated with CD31 expression in blood cells from patients with chronic obstructive pulmonary disease.
- Source :
-
Respiratory research [Respir Res] 2016 Oct 27; Vol. 17 (1), pp. 139. Date of Electronic Publication: 2016 Oct 27. - Publication Year :
- 2016
-
Abstract
- Background: Cigarette smoke induced oxidative stress has been shown to reduce silent information regulator 1 (Sirt1) levels in lung tissue from smokers and patients with COPD patients. Sirt1 is known to inhibit endothelial senescence and may play a protective role in vascular cells. Endothelial progenitor cells (EPCs) are mobilized into circulation under various pathophysiological conditions, and are thought to play an important role in tissue repair in chronic obstructive lung disease (COPD). Therefore, Sirt1 and EPC-associated mRNAs were measured in blood samples from patients with COPD and from cultured CD34 <superscript>+</superscript> progenitor cells to examine whether these genes are associated with COPD development.<br />Methods: This study included 358 patients with a smoking history of more than 10 pack-years. RNA was extracted from blood samples and from CD34 <superscript>+</superscript> progenitor cells treated with cigarette smoke extract (CSE), followed by assessment of CD31, CD34, Sirt1 mRNA, miR-34a, and miR-126-3p expression by real-time RT-PCR.<br />Results: The expression of CD31, CD34, Sirt1 mRNAs, and miR-126-3p decreased and that of miR-34a increased in moderate COPD compared with that in control smokers. However, no significant differences in these genes were observed in blood cells from patients with severe COPD compared with those in control smokers. CSE significantly decreased Sirt1 and increased miR-34a expression in cultured progenitor cells.<br />Conclusion: Sirt1 expression in blood cells from patients with COPD could be a biomarker for disease stability in patients with moderate COPD. MiR-34a may participate in apoptosis and/or senescence of EPCs in smokers. Decreased expression of CD31, CD34, and miR-126-3p potentially represents decreased numbers of EPCs in blood cell from patients with COPD.
- Subjects :
- Aged
Aged, 80 and over
Apoptosis
Cell-Free Nucleic Acids blood
Cell-Free Nucleic Acids genetics
Cells, Cultured
Cellular Senescence
Endothelial Progenitor Cells metabolism
Endothelial Progenitor Cells pathology
Female
Gene Expression Regulation
Genetic Markers
Humans
Male
MicroRNAs blood
MicroRNAs genetics
Middle Aged
Platelet Endothelial Cell Adhesion Molecule-1 genetics
Prognosis
Pulmonary Disease, Chronic Obstructive genetics
Pulmonary Disease, Chronic Obstructive pathology
RNA, Messenger blood
RNA, Messenger genetics
Sirtuin 1 genetics
Smoking adverse effects
Smoking blood
Smoking genetics
Platelet Endothelial Cell Adhesion Molecule-1 blood
Pulmonary Disease, Chronic Obstructive blood
Sirtuin 1 blood
Subjects
Details
- Language :
- English
- ISSN :
- 1465-993X
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Respiratory research
- Publication Type :
- Academic Journal
- Accession number :
- 27784320
- Full Text :
- https://doi.org/10.1186/s12931-016-0452-2