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Expression of Therapeutic Gene FCU1 Sensitizes Pancreatic Cancer Cells to 5-Fluorocytosine and Enhances the Cytotoxic Effect of 5-Fluorouracil.
- Source :
-
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2016 Oct; Vol. 161 (6), pp. 808-810. Date of Electronic Publication: 2016 Oct 26. - Publication Year :
- 2016
-
Abstract
- Hybrid therapeutic gene FCU1 gene was cloned into a lentiviral expression vector and the therapeutic effect of its expression was studied in three pancreatic cancer cell lines. Expression of FCU1 gene sensitized cells of two of three studied pancreatic cancer cell lines to 5-fluorocytosine. In addition, uracil phosphoribosyl transferase activity of the hybrid FCU1 protein increased sensitivity of transfected cells of all three studied pancreatic cancer cell lines to 5-fluorouracil, a standard chemotherapeutic agent.
- Subjects :
- Cell Line, Tumor
Cytosine Deaminase metabolism
Drug Resistance, Neoplasm
Flucytosine metabolism
Flucytosine pharmacology
Fluorouracil pharmacology
Gene Expression
Genetic Vectors chemistry
Genetic Vectors metabolism
Humans
Insulin-Secreting Cells metabolism
Insulin-Secreting Cells pathology
Lentivirus genetics
Lentivirus metabolism
Pentosyltransferases metabolism
Plasmids chemistry
Plasmids metabolism
Recombinant Fusion Proteins metabolism
Transduction, Genetic
Antineoplastic Agents pharmacology
Cytosine Deaminase genetics
Insulin-Secreting Cells drug effects
Pentosyltransferases genetics
Recombinant Fusion Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1573-8221
- Volume :
- 161
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Bulletin of experimental biology and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 27783293
- Full Text :
- https://doi.org/10.1007/s10517-016-3517-9