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Apocynin ameliorates pressure overload-induced cardiac remodeling by inhibiting oxidative stress and apoptosis.
- Source :
-
Physiological research [Physiol Res] 2017 Nov 24; Vol. 66 (5), pp. 741-752. Date of Electronic Publication: 2017 Oct 26. - Publication Year :
- 2017
-
Abstract
- Oxidative stress plays an important role in pressure overload-induced cardiac remodeling. The purpose of this study was to determine whether apocynin, a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, attenuates pressure overload-induced cardiac remodeling in rats. After abdominal aorta constriction, the surviving rats were randomly divided into four groups: sham group, abdominal aorta constriction group, apocynin group, captopril group. Left ventricular pathological changes were studied using Masson's trichrome staining. Metalloproteinase-2 (MMP-2) levels in the left ventricle were analyzed by western blot and gelatin zymography. Oxidative stress and apoptotic index were also examined in cardiomyocytes using dihydroethidium and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), respectively. Our results showed that abdominal aorta constriction significantly caused excess collagen deposition and cardiac insult. Treatment with apocynin significantly inhibited deposition of collagen and reduced the level of MMP-2. Furthermore, apocynin also decreased the NADPH oxidase activity, reactive oxygen species production and cardiomyocyte apoptotic index. Interestingly, apocynin only inhibited NADPH oxidase activity without affecting its expression or the level of angiotensin II in the left ventricle. In conclusion, apocynin reduced collagen deposition, oxidative stress, and inhibited apoptosis, ultimately ameliorating cardiac remodeling by mechanisms that are independent of the renin-angiotensin system.
- Subjects :
- Animals
Apoptosis physiology
Blood Pressure physiology
Heart Rate drug effects
Heart Rate physiology
Male
Oxidative Stress physiology
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species antagonists & inhibitors
Reactive Oxygen Species metabolism
Ventricular Remodeling physiology
Acetophenones pharmacology
Antioxidants pharmacology
Apoptosis drug effects
Blood Pressure drug effects
Oxidative Stress drug effects
Ventricular Remodeling drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1802-9973
- Volume :
- 66
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Physiological research
- Publication Type :
- Academic Journal
- Accession number :
- 27782741
- Full Text :
- https://doi.org/10.33549/physiolres.933257